丹参保护胰腺缺血再灌注损伤作用机制探析.doc

丹参保护胰腺缺血再灌注损伤作用机制探析 　【摘要】 目的 探讨丹参（SM）对大鼠胰腺缺血—再灌注（IR）损伤的作用及其机制。方法 夹闭大鼠腹腔动脉、肠系膜上动脉40 min，再灌注6 h 制备胰腺IR损伤模型，取SD大鼠36只，随机分为3组（n=12）：假手术组（Sham组）、缺血—再灌注组（IR组）、缺血—再灌注丹参保护组（SM组）。光、电镜观察胰腺腺泡细胞结构改变；测定血淀粉酶、脂肪酶含量，测定大鼠胰腺组织SOD活性、MDA含量。结果 SM组淀粉酶、脂肪酶含量较IR组显著降低(Plt;0.01)；SM组SOD活性较IR组显著升高，丙二醛含量较IR组显著降低 (Plt;0.01)。结论 丹参可能通过提高SOD活性、降低MDA含量对胰腺缺血再灌注损伤起保护作用。 【关键词】 胰腺； 缺血再灌注；丹参； 超氧化物歧化酶；丙二醛 　　Research on Mechanism of SM’s Protection against Rat’s Pancreatic Ischemiareperfusion Injury 　　Abstract： Objective To investigate the protective effects of salvia miltiorrhiza (SM) on the pancreatic ischemiareperfusion injury in rats and their possible mechanism.Methods Thirtysix SD rats were divided into three groups (n=12) at random: sham group, ischemiareperfusion (IR) group and SM group. The pathologic changes of pancreatic tissue were observed by optic and electron microscope. The plasma concentrations of amylase and lipase were measured. The level of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in pancreatic tissue were determined, too. Results The plasma concentrations of amylase and lipase were significantly lower in SM group than in IR group (Plt;0.01), and the activity of SOD in SM group were significantly higher than in IR group (Plt;0.01). The MDA level of SM group was significantly lower than that of IR group (Plt;0.01).Conclusion SM has a protective effect on the pancreatic ischemia-reperfusion injuries by enhancing the activity of SOD and decreasing the level of MDA. 　　Key words： pancreatic ischemia-reperfusion; salvia miltiorrhiza; superoxide dismutase; malondialdehyde 移植器官缺血—再灌注（IR）损伤是移植物丧失功能的主要原因，研究表明丹参对肝脏、肾脏等器官IR损伤有保护作用[1,2]，但有关丹参对胰腺IR损伤的作用尚未见报道，本实验研究了丹参对大鼠胰腺IR后超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量的影响, 探讨丹参对胰腺IR损伤的作用及其机制。 　　1 材料与方法 　　1.1 动物模型与分组 　　将雄性SD大鼠36只（体质量200~250 g，由徐州医学院实验动物中心提供）随机分为三组（n=12）：（1）Sham组，术前30 min 经股静脉分支缓慢注射生理盐水40 ml/kg；（2）IR组，断流前30 min 经股静脉分支缓慢注射生理盐水40 ml/kg；（3）SM组：断流前30 min 经股静脉分支缓慢注射丹参注射液（上海第一制药厂生产，注射液10 ml 含丹参生药10 g）6 g/kg（加入生理盐水稀释成40 ml/kg）。 0