丹参注射液对哮喘大鼠肺IL13及嗜酸性粒细胞趋化因子表达影响.doc

丹参注射液对哮喘大鼠肺IL13及嗜酸性粒细胞趋化因子表达影响 　 作者：王成国, 薛克营, 程立, 李威, 石明 【摘要】 目的:探讨丹参注射液抑制哮喘气道变应性炎症作用的分子机制。方法:30只SD大鼠随机分成正常对照组、哮喘组和丹参治疗组。HE染色行肺组织病理学检查，计数支气管肺泡灌洗液（BALF）中细胞总数并分类，SP法和RTPCR测定肺组织中IL13和嗜酸性粒细胞趋化因子（Eotaxin）表达。结果:丹参组肺组织炎性细胞浸润明显减少，BALF细胞总数及淋巴细胞、中性粒细胞和嗜酸性粒细胞(Eos)百分率较哮喘组明显减少(P＜0.05，P＜0.01)。哮喘组肺组织IL13和Eotaxin表达较正常对照组明显增加(P＜0.05)，丹参组IL13和Eotaxin表达较哮喘组明显减少(P＜0.05)。IL13和Eotaxin表达呈正相关（r=0.90，P＜0.01）。结论:丹参注射液可减轻哮喘大鼠气道炎症反应，其机制可能与降低哮喘大鼠肺内IL13和Eotaxin表达有关。 【关键词】 丹参注射液；哮喘；IL13；嗜酸性粒细胞趋化因子 Abstract: Objective To investigate the molecular mechanism of inhibitory effect of Danshen injection on allergic airway inflammation of asthma.Methods Thirty SD rats were randomly divided into control group,asthma group and Danshen injection group.Bronchoalveolar lavage fluid (BALF) was collected,and cytology analysis was performed.The lung tissue was harvested and stained with HE.Interleukin13 (IL13) and Eotaxin in lung tissue were measured by RTPCR and SP method of immunohistochemistry assay. Results Compared with the asthma group,less infiltration of inflammatory cells in lung tissues was observed in Danshen injection group.Total cell number,the percentage of lymphocytes,neutrophils and eosinophils in BALF of Danshen injection group were lower than that of asthma group (P﹤0.05,P﹤0.01).The expression of IL13 and Eotaxin in lung tissue of asthma group were higher than that of control group and Danshen injection group (P﹤0.05).The expression of IL13 was negatively correlated with Eotaxin (r=0.90, P﹤0.01).Conclision Danshen injection could suppress airway inflammation of asthmatic rats,probably by decreasing the expression of IL13 and Eotaxin in lung tissue of asthmatic rats. Key words:Danshen injection;Asthma;Interleukin13;Eotaxin 支气管哮喘是由多种炎症细胞、炎症介质和细胞因子参与的气道慢性炎症性疾病，其中白介素13（IL13）和嗜酸性粒细胞趋化因子（Eotxin）是致哮喘重要因素。丹参注射液治疗哮喘疗效明显，但其机制不明。本研究通过建立哮喘大鼠模型，检测其肺组织IL13和Eotaxin的表达，以探讨丹参注射液抑制支气管哮喘气道变应性炎症作用的分子机制。 1 材料与方法 1.1 实验动物和药物 健康雄性SD大鼠，体重（250±20） g，SPF级（同济医学院实验动物中心）。随机分为正常对照组、哮喘组和丹参治疗组，每组10只。