补益气血方剂对大鼠慢性难愈性创面血管内皮生长因子及血管生成的影响.doc

补益气血方剂对大鼠慢性难愈性创面血管内皮生长因子及血管生成的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：补益气血方剂对大鼠慢性难愈性创面血管内皮生长因子及血管生成的影响 1 1 材料与方法 2 2 结 果 6 3 讨 论 6 文2：补益气血方剂对大鼠慢性难愈性创面血管内皮生长因子及血管生成的影响临床医学论文 8 1 材料与方法 9 2 结 果 12 3 讨 论 13 参考文摘引言： 15 原创性声明（模板） 16 文章致谢（模板） 17 正文 补益气血方剂对大鼠慢性难愈性创面血管内皮生长因子及血管生成的影响 文1：补益气血方剂对大鼠慢性难愈性创面血管内皮生长因子及血管生成的影响 Objective: To explore the effects of reinforcing qi and nourishing blood recipes on vascular endothelial growth factor (VEGF) and the microvessel count (MVC)in granulation tissue in rats with chronic skin ulce。 Methods: A total of 90 male SD rats with back fullthickness skin lesion were used in this study. Except for the normal control group， the rats were injected with hydrocortisone for inducing chronic skin ulce， and were randomly pided into untreated group， Sijunzi Decoction group， Siwu Decoction group and Bazhen Decoction group. The time of wound healing was observed; immunohistochemical method and image analytical method were used to test the expressio of VEGF and MVC in granulation tissue of dermal chronic ulce in the rats。 Results: In the untreated group， the time of wound healing was significantly longer than that in the normal group (P Conclusion: Reinforcing qi and nourishing blood recipes can promote wound healing in rats through upregulating the expression of VEGF in granulation tissue of dermal chronic ulce， and inducing angiogenesis。 Keywords: dermal chronic ulcer; wound healing; qiblood reinforcing; vascular endothelial growth factor; rats 慢性难愈性创面是因各种原因导致的局部组织缺损，创面难以愈合且愈合后创面每因损伤而复溃，是外科常见病，多发病。我们在长期的临床实践中发现，补益气血方剂治疗慢性难愈性创面有较好的疗效。为进一步探讨补益气血方剂促进创面愈合的作用机制，开展了以下研究。 1 材料与方法 实验材料 实验动物 90只雄性SD大鼠，清洁级，2月龄，体质量200～250 g，由上海斯莱克实验动物有限责任公司提供，动物合格证号为医动字第02222号。 实验药物 四君子汤（《太平惠民和剂局方》）由党参9 g、白术9 g、茯苓9 g、甘草6 g组成，减压浓缩至浓度为 g/ml，大鼠用药剂量为生药 g/(kg·d)。四物汤（《太平惠民和剂局方》）由熟地黄12 g、当归9 g、白芍药9 g、川芎6 g组成，减压浓缩至浓度为 g/ml，大鼠用药剂量为生药 g/(kg·d)。八珍汤（《正体类要》）由党参9 g、白术9 g、茯苓9 g、甘草6 g、熟地黄12 g、当归9 g、白芍药9 g、川芎6 g组成，减压浓缩至浓度为 g/ml，大鼠用药剂量为生药 g/(kg·d