不同土炒白术中白术内酯Ⅲ和白术多糖含量比较.doc

不同土炒白术中白术內酯Ⅲ和白术多糖含量比较 陈鸿平 刘友平 刘承萍 李萍 （成都中医药大学 四川成都 610075） 摘要:目的　探讨不同辅料土炮制白术对其主要有效成分的影响，为辅料土的筛选提供依据。方法　采用同批药材，用5种不同品种的辅料土炮制；采用高效液相色谱法白术內酯-Ⅲ的含量，采用紫外分光光度法测定不同土炒白术中多糖的含量。结果　白术内酯-Ⅲ含量高低为：为赤石脂炒白术＞壁土炒白术＞灶心土炒白术＞窑土炒白术＞黄土炒白术＞生白术；多糖含量高低为：灶心土炒白术＞黄土炒白术＞赤石脂炒白术＞窑土炒白术＞壁土炒白术＞生白术。结论　白术经不同辅料土炒炮制后，白术內酯-Ⅲ和多糖的含量均有增高，但不同炮制品之间无显著差异。 关键词：白术； 土炒炮制；白术內酯Ⅲ；白术多糖 Content Comparision of Atractylode III and Soluble Polysaccharide Processed with Different Soils CHEN Hong-ping, LIU You-ping, LIU Cheng-ping, LI Ping (Chengdu University of TCM, chengdu 610075, China) Abstract: Object: To discuss on the effect on the main effective ingredients of processed Atratylodes (Baizhu) with different soils as excipient and provide the basis for screening soils as processing excipient. Methods: Atratylodes of the same batch are processed with 5 different kinds of soils. Content of Atractylode III is determined by HPLC and Soluble Polysaccharide by UV spectrophotometry in different processed Atratylodes. Results: The contents of Atractylode III in different processed Atratylodes areHalloysitum rubrum processed Atratylodes＞Wall soil processed Atratylodes＞kitchen range soil processed Atratylodes＞kiln soil processed Atratylodes＞loess processed Atratylodes＞raw Atratylodes; The contents of Soluble Polysaccharide in different processed Atratylodes are kitchen range soil processed Atratylodes＞loess processed Atratylodes＞ processed Atratylodes＞kiln soil processed Atratylodes＞Wall soil processed Atratylodes＞raw Atratylodes. Conclusion: the contents of both Atractylode III and Soluble Polysaccharide increased after soil processed. However, there is no significant difference among the processed Atratylodes with different soils. Key words: Atratylodes (baizhu), soil processing, Atractylode III, Soluble Polysaccharide 白术为菊科植物Atractylodes macrocephala Koidz．的干燥根茎。始载于《神农本草经》，为常用中药。具有健脾益气，燥湿利水，止汗，安胎等功效[1]。传统认为白术生品燥湿力强，炮制后可缓和药性，增强健脾和胃、补脾止泻的功效。白术历代炮制方法主要有熬制、土制、炒制、泔制、麸炒、醋制、煨制、焙制等，现代炮制方法多为清炒、土炒、麸炒、蜜炙麸炒等，其中土炒是最常见的炮制方法之一。白术土炒起源于