不同醋制方法对延胡索中延胡索乙素和原阿片碱含量的影响.doc

不同醋制方法对延胡索中延胡索乙素和原阿片碱含量的影响 宋平顺 赵建邦 （甘肃省药品检验所、甘肃省中药分析测试中心，兰州730000） 丁永辉 （甘肃省食品药品监督管理局） 赵燕（陇南市药品检验所 ） 摘 要 目的 探讨延胡索不同醋制方法对其延胡索乙素、原阿片碱含量的影响，结合对市售品炮制品的测定，制定醋延胡索的质量标准。方法 色谱柱：大连依利特 (250mm×4.6mm,5μm)；流动相为甲醇-0.6%冰醋酸（含0.6%三乙胺）(20：80)；检测被长为280nm；流速1.0ml/min；柱温：室温。结果 延胡索乙素、原阿片碱分别在0.245～1.467μg、0.202～1.008μg范围呈良好的线性关系，平均回收率分别为104.01％、101.22％，RSD分别为1.76％、1.45％(n=6)。醋延胡索中延胡索乙素、原阿片碱的含量在相对稳定范围之内。结论 方法准确，灵敏度高，重复性好，适用于延胡索炮制品的质量控制。制定醋延胡索中延胡索乙素、原阿片碱的含量是可行的。 关键词 延胡索；醋制品；延胡索乙素；原阿片碱；高效液相色谱法 Influence of different method of vinegar on the content of Tetrahydropalmatine and Opioid alkali in YanhusuoSong Pingshun Zhao Jianbang (Gansu Institute for Drug Control , analysis and testing of Gansu Province Traditional Chinese Medicine Centre, Lanzhou 730000) Ding Yonghui (Gansu Province Food and Drug Administration) Zhao Yan (Longnan Institute for Drug Control) Abstract Objective：To different method of vinegar on the content of Tetrahydropalmatine, opioid alkali in Yanhusuo, connected with the processed products on the market for the determination and make the quality standards of Yanhusuo, which is made by vinegar. Mtheod: column: Dalian according to Gbadolite (250 mm × 4.6mm, 5 μ m); mobile phase of methanol -0.6% acetic acid (with 0.6 percent triethylamine) (20:80); detection was the head of 280 nm; flow rate of 1.0 ml / min; column: room temperature. Results: The tetrahydropalmatine, former Argentine protopine in 0.245 ~ 1.467 μ g, 0.202 ~ 1.008 μ g of a good linear relationship, with an average recovery rate was 104.01%, 101.22%, RSD were 1.76%, 1.45% (n = 6 ). Conclusion: method is accurate, sensitive, reproducible, Corydalis apply to processed products quality control. Key words yanhusuo; vinegar products;-tetrahydropalmatine; former Argentine protopine HPLC. 延胡索是我国传统药物之一，《中国药典》2005年版收载为罂粟科植物延胡索Corydalis yanhusuo W.T．Wang的干燥块茎，具有活血、利气、止痛的功效[1]。延胡索生物碱类成分的测定已有报道,有重量法测定总生物碱[2]、紫外法测总生物碱[3、4、5]、电位滴定法测总生物碱[6、7]、薄层扫描法测定延胡索乙素[6]、高效液相法测定延胡索乙素[7]。同时测定延胡索(包括同属植物)中季胺碱[8]或叔胺碱[9