连续性静脉-静脉血液滤过患者血清中利奈唑胺的浓度监测.doc

连续性静脉-静脉血液滤过患者血清中利奈唑胺的浓度监测杨华1,3，董海燕1，董亚琳1* 1. 西安交通大学医学院第一附属医院药学部，陕西 西安 710061；2. 西安交通大学医学院第一附属医院中心ICU；3. 西安市红十字会医院，陕西 西安 710054[摘要] 目的：对连续性静脉-静脉血液滤过（continuous venovenous hemofiltration，CVVH)治疗的危重患者进行方法：高效液相色谱法患者血清中利奈唑胺结果：利奈唑胺在0.31～20.00(g·ml-1内线性关系良好（r = 0.9995），定量限为0.31 (g·ml-1，回收率为99.12％。5例危重患者治疗期间监测利奈唑胺浓度变化明显，变化范围为1.53-17.10 (g·ml-1。有2例患者谷浓度变化较大（相差近5倍）。结论：高效液相色谱法简单、快速、准确、灵敏、重现性好，可用于临床利奈唑胺的浓度监测。进行连续性静脉-静脉血液滤过治疗的患者，应用利奈唑胺时血药浓度变化较大，需要通过治疗药物监测的方法制定个体化给药方案。 [关键词] 利奈唑胺；高效液相色谱法；治疗药物监测；连续性静脉-静脉血液滤过R978.1 [文献标识码] A [文章编号] Determination of serum linezolid concentration in critically ill patients during continuous veno-venous hemofiltration YANG Hua1,3, DONG Hai-yan1, YOU Hai-sheng1, WANG Xue2, LI Hao2, DONG Wei-hua1，DONG Ya-lin1* ( 1.The First Affiliated Hospital, School of Medicine, Xi’an Jiaotong University,Shanxi Xi’an 710061; 2. The First Affiliated Hospital, ICU, Jiaotong University, Shanxi Xi’an 710061;3. Xi’an Red Cross Hospital, Shanxi Xi’an 710054) Abstract: Objective To monitor the serum linezolid trough concentration in critically ill patients with continuous venovenous hemofiltration (CVVH), in order to make individualized treatment regiment for these patients. Methods To establish a method of determining serum linezolid in human by HPLC (internal standard method). Result A good linearity was demonstrated between 0.31 – 20.00(g·ml-1 ( r = 0.9995), the lowest limit of quantification of 0.31(g·ml-1. The relative recovery was 99.12%. Linezolid serum concentrations changed significantly in the five patients during CVVH, with the trough concentration range of 1.53-17.10 (g·ml-1. The linezolid trough concentration in two patients showed changing greatly (nearly 5-fold). Conclusion The method is simple, rapid, accurate, sensitive, reproducible, and validated for determination of serum linezolid in patients. Linezolid serum concentrations in critically ill patients during continuous venovenous hemofiltration showed significant variability. Drug conce