毕业论文：环境工程论文翻译参考文献（终稿）.doc

Biofouling Vol. 26, No. 3, April 2010, 301–312 The uronic acids assay: a method for the determination of chemical activity on bio?lm EPS Kristina D.A. Mojica and Michael J. Cooney Department of Oceanography, School of Ocean and Earth Sciences and Technology, University of Hawaii, Honolulu, HI 96822, USA; bHawaii Natural Energy Institute, School of Ocean and Earth Sciences and Technology, University of Hawaii, Honolulu, HI 96822, USA (Received 7 June 2009; ?nal version received 20 November 2009) In this work, the uronic acids assay was evaluated for its potential to function as a bioassay to screen for antagonistic activity gainst the production of microbial bio?lm exopolysaccharide(EPS). The assay was ?rst applied to bio?lms produced in the presence of two universal disinfectants (sodium hypochlorite and sodium dodecyl sulfate) known o inhibit microbial growth and bio?lm formation. The performance of the assay was the characterized through statistical assessment of threshold concentrations for disinfection e?ciency and consistency relative to values reported in the literature. The assay was then evaluated for its utility in screening for enzymatic or chemical inhibitors of bio?lm formation (eg glycosidases, halogenated furanones, and semi-crude fractions extracted from minimally fouled marine plants) and its ability to distinguish between true anti-bio?lm activity and simple disinfection. Activity was characterized as (i) no e?ect, (ii) a true positive e?ect (ie increased bio?lm EPS), (iii) anti- bacterial activity (ie decreased bio?lm EPS and analogous decrease in planktonic growth), and (iv) anti-bio?lm EPS activity (ie decreased bio?lm EPS, without analogous decrease in planktonic growth). Results demonstrate that the uronic acids assay can augment existing bio?lm characterization methods by providing a quantitative measure of bio?lm EPS. Keywords: uronic acid; bio?lm; exopolysaccharide; assay; C. l