麻杏石甘汤抗流感病毒有效部位黄酮含量测定.docVIP

麻杏石甘汤抗流感病毒有效部位黄酮含量测定 摘要： 目的 建立麻杏石甘汤抗流感病毒有效部位群总黄酮含量的测定方法。方法 芦丁参照品和样品分别经过Al2(NO3)3-NaOH-NaNO2显色，采用紫外分光光度法在333nm波长处测定麻杏石甘汤总黄酮含量。结果 回归方程为Y=0.01417X-0.01763，相关系数为0.99918；精密 ... p摘要：nbsp; 目的nbsp; 建立麻杏石甘汤抗流感病毒有效部位群总黄酮含量的测定方法。方法 芦丁参照品和样品分别经过Al2(NO3)3-NaOH-NaNO2显色，采用紫外分光光度法在333nm波长处测定麻杏石甘汤总黄酮含量。结果nbsp; 回归方程为Y=0.X-0.，相关系数为0.；精密度高,重现性与稳定性较好。 结论nbsp; 应用紫外分光光度法测定麻杏石甘汤总黄酮含量准确度较高，是切实可行的含量测定方法。br / 关键词：麻杏石甘汤；黄酮；Al2(NO3)3-NaOH-NaNO2显色法；紫外分光光度法 /p pExperimenal study Of Maxingshigan Valid Part flavonoids its Polysaccharides on anti-Influenzabr / Abstract:Objectivenbsp; To establish the Method for Determination of Total Flavonoids in the Flavonoid Fraction of Maxingshigan dectoctin Antiviral Components. Methodsnbsp; The content of total flavonoids was determined with Al2(NO3)3-NaOH-NaNO2 reaction development process,and selected rutin as reference substance. Resultsnbsp; The regression equation was as follows: Y=0.X-0. (R2=0.);high preacute;cision preferably repeatability and stability. Conclusionnbsp; To determine content of flavonoids in rutin with ultraviolet spectrophotometry by standard Liquiritin is a practical method with higher accuracy.br / Key words: Maxingshigan dectoctin; flavonoid; Al2(NO3)3-NaOH-NaNO2 reaction development process; ultraviolet spectrophotometry /p pbr / 目nbsp; 录nbsp;nbsp; 7200字br / 摘要：nbsp;nbsp;nbsp; 1br / 关键词：nbsp;nbsp;nbsp; 1br / 1 前言nbsp;nbsp;nbsp; 1br / 1.1 研究麻杏石甘汤抗流感病毒总黄酮含量测定意义nbsp;nbsp;nbsp; 2br / 1.2nbsp;nbsp; 麻杏石甘汤提取精制工艺nbsp;nbsp;nbsp; 2br / 1.3nbsp;nbsp; 黄酮的分离纯化工艺nbsp;nbsp;nbsp; 3br / 1.4nbsp;nbsp; 黄酮的检测方法nbsp;nbsp;nbsp; 4br / 2nbsp; 实验部分nbsp;nbsp;nbsp; 5br / 2.1nbsp; 实验材料与仪器nbsp;nbsp;nbsp; 5br / 2.1.1 实验材料(包括药品、试剂、材料)nbsp;nbsp;nbsp; 5br / 2.1.2nbsp; 实验仪器nbsp;nbsp;nbsp; 5br / 2.2nbsp; 实验步骤nbsp;nbsp;nbsp; 5br / 2.2.1nbsp; 麻杏石甘汤的制备nbsp;nbsp;nbsp; 6br / 2.2.2nbsp; pH值梯度萃取法分离黄酮nbsp;nbsp;nbsp; 6br / 2.2.3nbsp; 紫外分光光度法测定总黄酮的含量nbsp;nbsp;nbsp; 6br / 2.2.4nbsp; 样品的测定nbsp;nbsp;nbsp; 7br / 2.2.5nbsp; 阴性样品的测定nbsp;nbsp;nbsp; 7br / 3nbsp; 讨论nbsp;nbsp;nbsp;