淀粉酶蛋白类抑制剂的研究进展.pdf

312 2011, Vol. 32, No. 09 食品科学 ※专题论述 α-淀粉酶蛋白类抑制剂的研究进展 魏鹏娟，王鲁峰，徐晓云，潘思轶* (华中农业大学食品科学技术学院，湖北武汉 430070) 摘 要：α- 淀粉酶的蛋白类抑制剂在控制内源性α- 淀粉酶和植物病虫害方面起着天然的防御作用；有一些还具有 一定的抗营养作用。本文简要综述α- 淀粉酶的蛋白类抑制剂分类，常用的分离纯化技术及其活性测定方法；其次 重点介绍和讨论其结构和作用机理；最后概述α- 淀粉酶抑制剂在病虫害防治及营养、健康和疾病防治方面的应用。 关键词：α- 淀粉酶蛋白类抑制剂；理化性质；抑制机理；应用 Research Progresses on Proteinaceous α-Amylase Inhibitors WEI Peng-j uan ，WANG Lu-feng ，XU Xiao-yun ，PAN Si-yi* (College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China) Abstract ：Proteinaceous α-amylase inhibitors play a natural role in the control of endogenous α-amylase activity and the defen se again st pests and pathogen s; some inhibitors were reported to have anti-nutritional function . In this review, the classification, common methods of purification and activity-determination, especially the structure and inhibitory mechanism of proteinaceous α-amylase inhibitors were introduced. The applications of α-amylase inhibitors in pest control, as well as in nutrition, heath, and disease prevention were also summarized. Key words ：proteinaceou s α-amylase inhibitor ；phy sicochemical property ；inhibitory mechanism ；application 中图分类号：Q8 16 文献标识码：A 文章编号：1002-6630(20 11)09-03 12-07 随着人们生活水平的提高和平均寿命的延长，无论 制剂的主要来源。有关淀粉酶抑制剂的研究迄今已经进 [4 ] 是发达国家还是发展中国家，肥胖、糖尿病等疾病患 行了70 余年，最早的报道是1933 年，Franco 等 从小 者都在逐年增加，减肥、降血糖等相关研究受到了越 麦中分离得到了蛋白质性质的淀粉酶抑制剂。能够抑制 来越高的重视。由于α- 淀粉酶抑制剂能有效抑制口腔 α- 淀粉酶的蛋白质可由微生物、