HPLC-PDA检测结合相对校正因子法测定爵床中7个木脂素成分的含量.doc

  Simultaneous determination of seven lignans in Justicia procumbens by high performance liquid chromatography-  5 10 15 20 25 30 35 40 photodiode array detection using relative response factors# LUO Zuliang1, KONG Weijun1, QIU Feng1, YANG Meihua1, LI Qian1, WEI Riwei1, YANG Xiaoli1, QIN Jieping2** (1. Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193; 2. Guangxi University of Chinese Medicine, NanNing 530001) Abstract: A simple and sensitive high performance liquid chromatography coupled with photodiode array (HPLC-PDA) method was developed for simultaneous determination of 7 lignans in Justicia procumbens using relative response factors. The chromatographic separation was performed on a Shiseido Capcell Pak C18 column (250 × 4.6 mm id, 5 μm), a gradient elution of acetonitrile/water and a photodiode array detector. The column temperature was maintained at 35 °C and the detection wavelength was set at 256 nm. Chinensinaphthol methyl ether was selected as the reference compound for calculating the relative response factors of the lignans. It has shown that the relative response factors (RRFs) for lignans are quite similar at 256 nm of detection under different analytical conditions (different columns and different HPLC instruments). Using RRFs, no lignan is needed as a reference standard, making the method ideal for rapid, routine analysis, especially for those laboratories where lignans standards are not readily available. An economic and practicable HPLC method using RRFs was established for the determination of seven lignans in J. procumbens. This method not only can determine multiple indexes in Chinese materia medica (CMM) simultaneously, but also resolve the problem of lacking of chemical standards. It will be a good quality evaluation method and pattern for CMM. Keywords: HPLC-PDA; Relative response factors; J. procumbens; Lignans 0 Introduction J. procumbens