ETAR结合肽耦联的Snail2 siRNA纳米粒抑制卵巢癌转移的体内外研究.doc

  ETAR 结合肽耦联的 Snail2 siRNA 纳米粒 抑制卵巢癌转移的体内外研究# 钟亚华1，张弓1，彭晋1，王全胜2，马欢1，张阿丽1** 5 10 15 20 25 30 35 40 （1. 武汉大学中南医院放化疗科，武汉 430071； 2. 华中科技大学协和医院中医科，武汉 430000） 摘要：Snail2 （SLUG）通过抑制 E-cadherin, 在介导上皮性癌细胞发生上皮间质细胞转型 （EMT）中发挥了重要的作用。 内皮素-1（ET-1）和内皮素受体（ETAR）在卵巢癌原发灶和 转移灶中表达量高。而且，在卵巢癌患者的腹水中可以检测到 ET-1 高表达，表明 ET-1 可 以促进肿瘤的播散。本文研究 ETAR 结合肽耦联的 Snail2 siRNA 纳米粒抑制卵巢癌转移的体 内外作用。我们发现，体外实验中，ETAR 结合肽耦联的 Snail2 siRNA 纳米粒能促进 E-cadherin 的表达，而降低 Vimentin 的蛋白表达；Boyden 小室体外侵袭实验表明 ETAR 结 合肽耦联的 Snail2 siRNA 纳米粒能抑制卵巢癌细胞的体外侵袭；建立卵巢癌转移的皮下和 腹腔模型，给予 ETAR 结合肽耦联的 Snail2 siRNA 纳米粒处理，结果发现卵巢癌皮下瘤的生 长减慢同时，卵巢癌腹腔肿瘤瘤的腹腔转移和腹水的生成减少。这些结果表明 ETAR 结合肽 耦联的 Snail2 siRNA 纳米粒能抑制卵巢癌的体内外转移潜能。 关键词：SLUG；EMT；卵巢癌 中图分类号：R737 Snail2 siRNA narnoparticle combined with ETAR peptide inhibit the metastasis ability of ovarian tumor in vitro and in vivo Zhong Yahua1, Zhang Gong1, Peng Jin1, Wang Quansheng2, Ma Huan1, Zhang Ali1 (1. Zhongnan Hospital,Wuhan University, WuHan 430071; 2. Union Hospital of Huazhong University of Science and Technology, WuHan 430000) Abstract: Abstract: Snail2(SLUG) plays an important role in epithelial to mesenchymal transition(EMT) of the epithelial cancer cells. Endothelin-1 and the receptor ETAR have the high expression in the primary tumor in ovarian cancer, at the same time, there are have high level of ET-1 in the ascites of ovarian tumor patients which indicated that ET-1 can promote tumor metastasis. Our study try to observe the inhibited role of Snail2 siRNA narnoparticle combined with ETAR peptide in ovarian tumor in vitro and in vivo. We found Snail2 siRNA narnoparticle combined with ETAR peptide complex can induce E-cadherin expression and decrease Vimentin expression; this complex can decrease the in vitro invasion ability of ovarian tumor cells ,and also the complex can inhibit the tumor growth and ascites production in ovarian tumor mice models. Results show that Snail2 siRNA narnoparticle combined with ETAR peptide complex can inhibit the metastasis ability of ovarian tumor in vitro and in