关于水稻胚乳淀粉体发育的研究.docVIP

  Fibroblast activity on multilayer laminin5 beta3 DNA coating titanium surface JIANG Qiaohong, ZHAO Shifang, GONG Xue, HE Fuming, YANG Guoli** 5 10 15 (The Affiliated Stomatology Hospital, School of Medical, Zhejiang University, HangZhou 310006) Abstract: This work was to investigate the fabrication of multilayer gene-functionalized films composed of cationic liposome (Lip) and pEGFP-C1-LAMB3 (pGL) DNA on titanium surfaces and the effect of the gene coatings on NIH3T3 cells. The multilayer gene coatings were fabricated on titanium by layer-by-layer (LbL) assembly technique. Surfaces analysis was carried out by contact angle measurements and X-ray photoelectron spectroscopy (XPS). Formation of LBL films was confirmed by fluorescence microscope. A biological study with NIH3T3 was followed over a 7-day culture period at days 0, 3 and 7 to observe the viability. This study showed polyelectrolyte multilayer films could be successfully deposited. EGFP expression increased with adding layer numbers. NIH3T3 adhered on multilayered DNA coatings displayed significantly higher (P0.05) cell viability compared to control group both for 3 and 7 days culture. The results indicated the HA/pGL coatings improve the cytocompatibility of native titanium surface and presents a promising platform for delivery of DNA to NIH3T3 cells. Keywords: Transfection; Gene; Laminin5; Liposome; Titanium 20 0 Introduction Infection originating from soft tissues may potentially progress deeper into bone tissue and undermine implant osseointegration. Attachment loss of soft tissues around dental implants is one of the most important reasons resulting in implant failures. Failure of dental implants after 25 30 35 40 45 long-term implantation (more than 10 years) has been shown to be caused by a bacterial infection in up to 30% of cases [1]. A well soft tissue-implant biologic seal may prevent oral bacteria and their products from penetrating into the body, so it is very important to form an earl