河北省棉花黄萎病菌致病性与ISSR遗传分化.docVIP

  河北省棉花黄萎病菌致病性与 ISSR 遗传 分化# 王国宁1，赵贵元2，岳晓伟1，李志坤1，张艳1，张桂寅1，吴立强1，王省芬1， 5 10 15 20 25 30 35 40 45 马峙英1** （1. 河北农业大学河北省作物种质资源重点实验室，河北 保定 071001； 2. 河北省农林科学院，石家庄 050000） 摘要：从河北省 17 个主要植棉县采集棉花黄萎病株，分离获得 52 个黄萎病菌单孢菌系，对 其培养特性、致病性和 ISSR(inter-simple sequene repeat)遗传分化进行了研究。菌系培 养性状研究表明，在采集的 52 个菌系中，菌核型菌系最多，其次是菌丝型，最少的是中间 型，三种类型菌系分别占总菌系的 51.9%，38.5%和 9.6%。利用 7 个抗、感不同的鉴别寄主 在光、温、湿可控的生长室鉴定了病菌的致病性，供试菌系可分为致病力强、中、弱三种类 型(型、型和型)，分别占供试菌系的 51.9%，21.2%和 26.9%。在供试菌系中存在比落 叶型菌系致病力还要强的非落叶型菌系。基于病情指数的聚类分析结果表明，河北省棉花黄 萎病菌系存在明显致病力分化，但与地理来源无关。菌核型菌系和中间型菌系多表现为强致 病力或中等致病力，而菌丝型菌系的致病力变化较大。在 136 个 ISSR 标记中，80 个属于多 态性标记，多态性位点百分率达 58.8%。基于 ISSR 的聚类分析结果表明，河北省棉花黄萎 病菌的遗传分化较小，并且遗传分化与地理来源相关。 关键词：棉花；黄萎病菌；培养性状；致病力；ISSR；遗传分化 中图分类号：S435.621 Pathogenicity and ISSR genetic differentiation of Verticillium dahliae isolates from cotton growing areas of Hebei province WANG Guonig1, ZHAO Guiyuan2, YUE Xiaowei1, LI Zhikun1, ZHANG Yan1, ZHANG Guiyin1, WU Liqiang1, WANG Xingfen1, MA Zhiying1 (1. Key Laboratory of Crop Germplasm Resources of Hebei Province,Agricultural University of HeBei BaoDing 071001; 2. Hebei Academy of Agricultrue and forestry Sciences, ShiJiaZhuang 050000) Abstract: 52 Verticillium dahliae isolates were recovered from 17 main cotton growing counties of Hebei Province. Culturing characteristics, pathogenicity and genetic variation based on ISSR marker were studied. According to culturing characteristics on PDA medium, the tested isolates could be classified into sclerotia type, filamentous type and intermediate type. Sclerotia type isolates which accounted for 51.9% were the most, followed by filamentous type (38.5%) and intermediate type (9.6%). In the growth chamber where the light, temperature and humidity were controlled automatically, the pathogenicity of 52 isolates was evaluated on eight cotton varieties with different disease resistance to Verticillium wilt. The results indicated that the isolates could be divided into three pathogenic types, i.e. strong (typeⅠ), mild (type Ⅱ) and weak (type