2008-2010年中国部分马铃薯主产区致病疫霉群体基因型结构的研究.docVIP

  2008-2010 年中国部分马铃薯主产区致病疫 霉群体基因型结构的研究# 宋俊丽，杨志辉，朱杰华** 5 10 15 20 25 30 35 （河北农业大学植物保护学院，河北 保定 071001） 摘要：【目的】通过测定 2008-2010 年中国部分马铃薯主产区致病疫霉群体基因型，揭示其 群体遗传结构和变化。【方法】利用 PCR-RFLP 技术检测致病疫霉线粒体 DNA 单倍型，采 用 SSR 和 AFLP 标记确定致病疫霉群体的基因型。【结果】89 株供试致病疫霉中，共监测 到 Ia、IIa 和 IIb 型 3 种线粒体 DNA 单倍型。其中，Ia 型 57 株，占 64.04%，是优势谱 系，分布在宁夏、云南和四川。在 Pi04、Pi33、Pi63、Pi56 和 Pi70 五个 SSR 基因座上 共检测到 11 个等位基因，其中基因座 Pi70 中的 192 和基因座 Pi33 中的 203 为优势等位基 因，基因频率分别是 99.50%和 88.12%,共鉴定出 8 种 SSR 基因型。AFLP 聚类在遗传相似系 数接近 0.714 时，形成 A、B 两组，所有 A1 交配型菌株分布在 A 组，A2 交配型和自育型 菌株分布在 B 组。【结论】致病疫霉 SSR 基因型与交配型间没有明显相关性，AFLP 基因 型与交配型有一定的相关性，而与线粒体 DNA 单倍型和 SSR 基因型间没有明显相关性。我 国致病疫霉群体遗传多样性程度不高。 关键词：致病疫霉；线粒体 DNA 单倍型；简单重复序列；扩增片段长度多态性；基因型 中图分类号：S435 Population Genotypic Structure of Phytophthora infestans in Major Potato Producing Areas of China SONG Junli, YANG Zhihui, ZHU Jiehua (College of Plant Protection, Agricultural University of Hebei, HeBei BaoDing 071001) Abstract: [Objective] The genetic diversity of Phytophthora infestans populations collected from 2008 to 2010 was revealed by genotype detection. [Methods] Mitochondrial DNA haplotyple was detected by means of restriction fragment length polymorphism-polymerase chain reaction (PCR-RFLP). Genotypes were assessed for simple sequence repeats (SSR) and amplified fragment length polymorphism (AFLP). [Results] Amomg 89 isolates of P. infestans on potato, Ia, IIa and IIb were detected. Ia haplotype, 57 isolates, were advantage lineage. The frequency of Ia haplotype was 64.04%, spreading in Ning xia, Yun nan and Si chuan. Eleven alleles were detected on five SSR gene locus Pi04, Pi33, Pi63, Pi56 and Pi70 in P.infestans populations. The 192 and 203 located on gene locus Pi70 and Pi33 were predominant SSR ones among eleven alleles and gene frequency was 99.50% and 88.12%, respectively. Total eight different SSR genotypes were detected among 89 isolates collected from potato. [Conclusion] SSR genotype of P. infestans isolates were not correlate with mating type. AFLP genotype of P.