白藜芦醇对高脂日粮小鼠肌肉氧化还原状态及蛋白质代谢的影响.docVIP

  白藜芦醇对高脂日粮小鼠肌肉氧化还原状 态及蛋白质代谢的影响# 李翔辉1，白静1，唐雪1，施用晖1,2，乐国伟1,2** 5 10 15 20 25 30 35 40 45 （1. 江南大学食品学院，食品营养与功能因子研究中心，江苏 无锡 214122； 2. 江南大学食品科学与技术国家重点实验室，江苏 无锡 214122） 摘要：目的 研究白藜芦醇对长期高脂日粮诱导肥胖型大鼠肌肉组织氧化还原状态的调节作 用以及对蛋白质代谢调节作用可能的机制。方法 4 周龄昆明小鼠随机分为正常日粮饲喂的 对照组，高脂日粮饲喂的高脂组，12 周后选取高体重小鼠分为三组，分别饲喂添加 0%、0.03% 和 0.06%白藜芦醇的高脂日粮 18 周。结果 高脂组相比对照组肌肉组织抗氧化酶活力显著降 低，MDA 含量显著增加，腿肌率显著降低，腹脂率显著增加(P0.05)；核糖体翻译能力显著 降低(P0.05)；蛋白合成关键基因 mTOR，肌细胞生成素(Myogenin)mRNA 表达水平显著提高 (P0.05)，肌抑素(Myostatin)，蛋白质降解关键基因泛素蛋白(Ubipuitin protein)mRNA 表达水平显著降低(P0.05)。添加不同剂量白藜芦醇组均能显著改善小鼠肌肉组织氧还原状 态(P0.05)，显著提高腿肌率，核糖体翻译能力，mTOR，Myogenin 基因 mRNA 表达水平 (P0.05)，显著降低 Myostatin，Ubipuitin protein 基因 mRNA 表达水平(P0.05)，添加 0.06%效果更显著。结论 添加适量白藜芦醇能改善高脂日粮诱导肥胖小鼠肌肉组织氧化还原 状态，提高腿肌率，可能是白藜芦醇通过改善氧化还原状态改善了蛋白质代谢。 关键词：白藜芦醇；高脂日粮；肌肉；蛋白质代谢；氧化还原状态 中图分类号：R151.1 Effects of resveratrol on redox state ,protein metabolism in muscle of long-term high-fat diet fed mice LI Xianghui1, BAI Jing1, TANG Xue1, SHI Yonghui1,2, LE Guowei1,2 (1. Jiangnan University，School of Food Science and Technology，The Research Center of Food Nutrition and Functional Factors, JiangSu WuXi 214122; 2. The State Key Laboratory of Food Science and Technology Jiangnan University, JiangSu WuXi 214122) Abstract: Purpose The purpose was to study the effect of resveratrol on the rat muscle tissue redox state regulation of long-term high-fat diet-induced obese and the possible mechanisms of protein metabolism.Method 4-weeks-old mice were randomly divided into control group fed with normal diet, the high fat group fed with high-fat diet, 12 weeks later，selected the high weight mice and divided them into three groups that were fed with 0% 0.03% and 0.06% resveratrol high-fat diet for 18 weeks separately.Results Compared to the control group, antioxidant enzyme vitality of the high-fat group muscle organization decreased significantly, the content of MDA increase significantly, leg muscle rate decreased significantly, the percentage of abdominal fat increased significantly(P0.05), riboso