恶性胸腔积液中Th17_Treg细胞的失衡.docVIP

  Th17/Treg imbalance in malignant pleural effusion# ZHOU Qiong, YANG Weibing, YE Zhijian, XIANG Fei, ZHANG Jianchu* (Department of Respiratory Diseases, Union Hospital, Tongji Medical College, Huazhong 5 10 15 20 25 30 35 University of Science and Technology,Wuhan 430022) Abstract: In this paper, the objective was to investigate the distribution of Th17 cells in relation to Tregs, as well as to Th1/Th2 balance in malignant pleural effusion. The numbers of Th17, Tregs, Th1, and Th2 cells in malignant pleural effusion and peripheral blood were determined by flow cytometry. The relationship among the numbers of Th17, Tregs, Th1, and Th2 cells were explored. Results showed that the numbers of Th17, Tregs, Th1, and Th2 cells were all increased in malignant pleural effusion when compared with the corresponding peripheral blood. Th17 cell numbers were correlated negatively with Tregs in malignant pleural effusion, but not in blood. Th17 cells and Th17/Treg ratios were positive, and Tregs were negative, correlated with Th1 cells, but not with either Th2 cells or Th1/Th2 ratios in malignant pleural effusion. This study supports earlier data that both Th17 cells and Treg are present at higher frequencies in malignant pleural effusions compared with the autologue blood. For the first time, we show that Th17/Treg imbalance exists in malignant pleural effusions. Key words: malignant pleural effusion; regulatory T cells; Th17 cells; Th1/Th2 imbalancekey 0 Introduction The development of inflammatory process in the pleural space may result in increased pleural vascular permeability, leading to the accumulation of fluid enriched in proteins and to the recruitment of cells with the development of exudative pleural effusion[1]. It has been well documented that CD4+ T lymphocyte dominance occurs in malignant pleural effusion (MPE) [1,2]. On encountering an antigen, na?ve CD4+ T-helper precursor cells enact a specific process that results in differentiation toward the T-helper ty