细胞因子信号传导抑制蛋白1对糖尿病小鼠肾损伤的实验研究.docVIP

  细胞因子信号传导抑制蛋白 1 对糖尿病小鼠 肾损伤的实验研究# 刘青娟1，邢玲玲2，刘淑霞3，段惠军3** 5 10 15 20 25 30 35 （1. 河北医科大学病理教研室，石家庄 050017； 2. 河北医科大学第二医院，石家庄，050000； 3. 河北医科大学病理教研室，石家庄，050017） 摘要：目的：探讨细胞因子信号传导抑制蛋白 1（Suppressors of cytokine signaling, SOCS-1） 在糖尿病大鼠肾组织炎性损伤中的作用。方法：雄性 CD-1 小鼠随机分为四组，对照组、糖 尿病组、SOCS-1 质粒转染组和空载体转染组。腹腔注射链脲佐菌素（Streptozotocin, STZ） 诱发糖尿病小鼠模型，转染组在成模后给予尾静脉注射 SOCS-1 质粒或空质粒载体，每隔 7 天注射一次。4 周后收集标本，检测各组动物的血糖、24h 尿蛋白；Western 蛋白印迹法检 测肾组织中 SOCS-1 蛋白的水平。流式细胞术检测肾组织中巨噬细胞标志蛋白 CD68 的水平； ELISA 检测肾组织中白细胞介素 -1β（ Interleukin-1β , IL-1β）和转化生长因子 -β 1 （Transforming growth factor-β1, TGF-β1）的水平。结果：过表达 SOCS-1 能抑制糖尿病大 鼠肾组织中 CD68、IL-1β和 TGF-β1 的表达、降低糖尿病大鼠尿蛋白排泄，但对血糖无影 响。结论：过表达 SOCS-1 能通过减轻糖尿病大鼠肾组织炎症反应从而缓解肾损伤。 关键词：糖尿病肾病；SOCS-1；炎症；IL-1β 中图分类号：R364 Role of SOCS-1 on diabetic renal injury LIU Qingjuan1, XING Lingling2, LIU Shuxia3, DUAN Huijun4 (1. Department of Pathology,Hebei Medical University, Shijiazhuang,050017; 2. The second hospital of Hebei Medical university,Shijiazhuang,050000; 3. Department of Pathology,Hebei Medical university, Shijiazhuang,050017; 4. Department of Pathology,Hebei Medical University,Shijiazhuang,050017) Abstract: Objective To investigate the role of SOCS-1 on diabetic renal injury. Methods Male CD-1 mice were randomly divided into four groups: control group, diabetic group, empty plasmid vector transfection group. SOCS-1 plasmid transfection group. Mice received an intraperitoneal injection of STZ at a dose of 150mg·kg-1 body weight. Transfection mice received an injection of SOCS-1 plasmid or empty vector and every 7 days thereafter. At 4 weeks after STZ injection, specimens were collected to detect the blood glucose and 24 hours urine protein. Western blotting analysis were used to determine the expression of SOCS-1. The expression of CD68 was detected by flow cytometry. The secretion levels of IL-1β and TGF-β1 were detected by ELISA. Results Overexpression of SOCS-1 in kidney ameliorated excretion of urine protein and the expression of CD68, IL-1β and TGF-β1. Conclusion