盐胁迫下抗虫棉与常规棉的比较转录组分析.docVIP

  Comparative transcriptome analysis between insect-resistant cotton and non-transgenic cotton 5 10 15 20 25 30 35 40 45 (Gossypium hirsutum L.) in response to salt stress# Fangjiao Xu1, Mingliang Jia2, Junli Feng1, Ying Liang1, Yanjun Liang1, Jishuang Chen2** (1. Institute of Bioengineering, Zhejiang Sci-Tech University, Hangzhou, 310018, Zhejiang, China; 2. College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 210009, Jiangsu, China) Abstract: Insect-resistant cotton is one of the main transgenic crops in the world. High salinity immensely limits insect-resistant cotton growth and productivity. To determined genes in response to salt stress and clarify reasons of transcription level changes caused by insect-resistant factor, gene expression microarray was used to monitor the differentially expressed genes in insect-resistant cotton CCRI 41 and non-transgenic cotton CCRI 23. Microarray analysis showed that 2341 genes were up-regulated and 3073genes were repressed in CCRI 41 exposed for 24 h to 200 mM NaCl. A total of 7674 salt-resistant genes of CCRI 23 were identified, the expression levels of 2341 genes were enhanced, and the transcript levels of 4718 genes were decreased by 200 mM NaCl. Gene ontology of annotated genes revealed that some of the salt responsive genes involved to important processes as the response to osmotic stress, abscisic acid stimulus and reactive oxygen species were induced by the NaCl treatment. Comparative transcriptome analysis between CCRI 41and CCRI 23 in response to salt stress exhibited that toxin catabolic process and regulation strategy of DNA level played important role in response to salt stress. Quantitative RT-PCR was used to validate 4 selected salt responsive genes. This work represents the first study in disclosing the possible molecular evidence that insect-resistant cotton have weak tolerance against NaCl stress. Comparative transcriptome analysis will assist in laying a fou