生物制药毕业论文--梨小食心虫线粒体基因全分析及进化树的构建.docVIP

梨小食心虫线粒体基因全分析及进化树的构建 摘 要伴随着生物信息学的发展，现在越来越多的学科接受了生物信息学的分析手段和分析方法，并将生物信息学的分析结果作为验证实验成果的有力依据梨小食心虫的危害也越来越突出，并且梨小食心虫的研究也已经进入了分子研究阶段，非常需要生物信息学的科学分析方法和分析结论，为其分子水平的结果有力论证。本论题的研究主要内容为运用生物信息学的各种成熟软件对从NCBI数据库中获取的梨小食心虫体内的线粒体基因全序列进行生物学分析，重点对基因的数列 外显子信息 编码蛋白及其理化性质进行分析，并预测其编码蛋白的结构与功能，并通过与代表性的生物进行同源进化树的构建通过实验论证，我们得出NC 014806，基因全长为15717bp，为一环形的双链DNA，存在一个长552bp的开放阅读框，编码为183个氨基酸，其翻译的蛋白质为酸性蛋白，亲水性较强，且以α–螺旋肽链盘绕为主，其序列与日本的野桑蚕线粒体有很高的同源性。经过多序列比对，构建进化树，我们可以梨小食心虫与茶小卷叶蛾朝灰蝶进化关系关系最近。关键词：梨小食心虫线粒体基因序列进化树 The complete analysis of Grapholita molesta mitochondrial DNA and the construction of the evolutionary tree Abstract: With the development of bioinformatics, more and more subjects accept of the tools and analytical methods of bioinformatics, and the result of bioinformatics analysis can be as a strong basis for the verification. Grapholitha molesta’s hazards has become increasingly prominent, and Grapholitha molesta’s research has entered a phase of molecular studies, a great need for the scientific analysis methods and conclusions of the bioinformatics, make a strong argument for the results of its molecular.The main content of this study is to use a variety of sophisticated bioinformatics software to analysis the mitochondrial gene sequences of the pear borer obtained from the NCBI database , focusing on the gene sequence, exon information,coding proteins and their physical and chemical properties, and forecast its encoded protein structure and function, to expect the valuable biological data obtained from the mass of biological data, and to construct the evolution tree through the compare with the representation of the biology. Through experiments, we can know that the Grapholitha molesta mitochondrial gene sequences is circular double-stranded DNA, the NCBI accession number for the NC 014806, Full-length is 15717bp,and between 6748 and 7299 exist of a long open reading frame of 552bp, encoding 183 amino acids,whose translation of the protein is acidic protein, a strong hydrophilic