生物科学专业毕业论文--绵羊MHC ClassⅡ基因的生物信息学分析.docVIP

摘 要 从NCBI中获取绵羊（Major Histocompatibility Complex，MHC，应用生物信息学,对绵羊MHC分子疏水区、跨膜区、结构功能域和生物活性位点等几方面生物学特性进行了分析，，，绵羊MHC分子有一个疏水区和跨膜区；该分子有主要组织相容性复合物蛋白免疫球蛋白信号及N-糖基化位点、蛋白质激酶C磷酸化位点等多个生物活性位点。绵羊MHC classABSTRACT Extract Ovis aries from NCBI of nucleotides and Major Histocompatibility Complex class II amino acid sequence, application bioinformatics principle, on Ovis aries MHC class II molecules succoth watershed and transmembrane area, the structure and function domain and biological activity sites aspects of biology characteristic was analyzed to predict the secondary structure and level 3 genetic structure, and the Ovis aries and other animals will be more nucleotide sequence of amino acid sequence and contrast, make more sequence phylogenetic tree. Results show that, Ovis aries MHC class II molecules have a dredging watershed and transmembrane area, the molecules are major histocompatibility complex protein immunoglobulin signal and N - glycosylation sites, protein kinase C phosphorylation sites such multiple bioactive loci sites, Ovis aries MHC class II the sequence of amino acids and nucleotide sequences and the Capra hircus is highest homology of 66% and 73%. Secondly Bubalus bubalis and Bos indicus homology of higher, with the lowest, homology of Larimichthys crocea for 25% and 51% respectively. Keywords: Major Histocompatibility Complex class II molecules; Bioinformatics; Sequence analys 目 录 第一章 前言 1 1.1 生物信息学简介 1 1.2 MHC的相关简介 1 第二章 材料来源 4 第三章 分析方法 5 3.1 蛋白质序列的获取 5 3.1.1 绵羊MHC ClassⅡ基因的核酸和蛋白质序列的获得 5 3.1.2 同源序列获得 5 3.2 蛋白质的基本性质分析 5 3.2.1 疏水性分析 5 3.2.2 跨膜区分析 5 3.2.3 功能结构域分析 5 3.2.4 生物活性位点分析 5 3.3 蛋白质结构预测 5 3.3.1 蛋白质二级结构的预测 5 3.3.2 蛋白质三级结构预测 5 3.4 绵羊MHC ClassⅡ系统进化树分析 5 第四章 结果与分析 6 4.1 绵羊MHC ClassⅡ基因的核酸和蛋白质序列 6 4.2 绵羊MHC ClassⅡ蛋白质的疏水性和跨膜区分析 7 4.2.1绵羊MHC ClassⅡ蛋白质的疏水性分析 7 4.2.2绵羊MHC ClassⅡ蛋白质的跨膜螺旋区分析 8 4.3 绵羊MHC ClassⅡ蛋白质的功能结构域分析 9 4.4 绵羊MHC ClassⅡ蛋白质生物活性位点分析 11 4.5 绵羊MHC ClassⅡ蛋白质的二级结构预测 12 4.6 绵羊MHC ClassⅡ蛋白质的三级结构预测 14 4.7 同源序列对比和系统发生分析 15 第五章 结论 21 参考文献