食品营养与检测专业毕业论文--转基因抗虫水稻中重金属铅、镉、铜、铬、铁含量的测定.docVIP

转基因抗虫水稻中重金属铅、镉、铜、 铬、铁含量的测定 摘要：本实验对转BT抗虫品种的转基因水稻进行湿法消解预处理，处理液采用石墨炉和火焰原子吸收光谱法进行检测，测定重金属铅、镉、铜、铬、铁的含量，并做加标回收实验。按照相同方法处理非转基因水稻，并测定其重金属的含量，通过比较，发现该品种的转基因水稻与非转基因水稻中这五种重金属的含量没有明显差别。 关键词: 转基因水稻 ；重金属；原子吸收光谱法 Determination of Pb, Cd, Cu, Cr and Fe in Transgenic insect-resistant rice by Atomic Absorption Spectrometry Abstract ：A simple procedure was developed to determine contents of heavy metals in BT to transgenic insect-resistant rice varieties. After wet digestion of samples, five heavy metals, Such as Pb, Cd, Cu, Cr and Fe were determined by flame atomic absorption spectrometry (FAAS)or graphite furnace atomic absorption spectrometry (GFAAS) with regard to their concentrations, respectively. The experimentsof standard addition were carried out to validate the proposed method, and good recoveries are present. According to theobtained concentrations of Pb, Cd, Cu, Cr and Fe in the transgenic rice and the non-transgenic rice, a conclusion canbe made that there is no obvious difference in the contents of heavy metals between the two types of tested samples. Key word: Transgenic rice ; heavy metals；atomic absorption spectrometry 目 录 摘要…………………………………………………………………………………Ⅰ 关键词………………………………………………………………………………Ⅰ Abstract…………………………………………………………………………… Ⅱ Keywords……………………………………………………………………………Ⅱ 绪论 1 1转BT抗虫品种的转基因水稻的介绍和研究现状..................... 1 1.1转BT抗虫品种的转基因水稻的介绍 1 1.2转基因抗虫水稻的研究进展 2 2重金属检测方法研究 4 2.1样品前处理..................................................4 2.2检测方法....................................................5 3本研究的目的意义..............................................8 1 实验部分 9 1.1仪器和试剂 9 1.1.1仪器 9 1.1.2试剂和材料 9 1.2 实验方法 9 1.2.1原料处理 9 1.2.2样品测定条件 9 1.2.3样品预处理 11 1.2.4工作曲线的绘制 12 1.2.5样品测定 12 1.2.6数据分析 12 2实验结果与分析 12 2.1测定条件的优化.............................................12 2.1.1铅、镉、铜和鉻测定条件的优化............................... 12 2.1．2铁测定条件的优化.........................................14 2.2共存离子干扰...............................................15 2.3检出限及精密度......................................