生物科学毕业论文设计-银杏内生真菌分离培养及生物活性测定.docVIP

化学工程与食品科学学院 毕 业 论 文（设 计） 题 目： 银杏内生真菌的分离培养与生物活性测定 专 业： 生物科学 班 级： 0911班 学生姓名： 岳继萍 学 号： 2009124121 指导老师： 阎 华 二〇一三 年 五 月 摘 要 内生真菌是一类生长于活体植物组织中的特殊的真菌，研究相对较少，生长于独特环境中，具有药用价值~树龄长或有地域特色的植物中一般都能分离出新的内生菌，而新种或罕见菌种又能为寻找新型生物活性物质提供前景.银杏是我国特色珍稀植物，具有。活化石。之美称。银杏在生长期间很少感染病虫害，寿命极长，因此具有很好的挖掘新型内生真菌的潜力，本研究采集湖北省襄阳市湖北文理学院内银杏枝条和树叶，采用组织分离法分离其中的内生真菌，筛选具有杭菌性和抗氧化活性的菌株并通过形态特征进行初步鉴定；对其中三株株杭菌活性显著且稳定的不产孢菌进行形态学研究和分子解析，对培养条件和培养基组成进行优化，进而分离其发酵产物中的抗菌活性物质并进行结构鉴定，为进一步工业化生产应用提供工作基础和依据。 银杏， 又名白果， ， ， ， ， ， (外种皮)及果仁的提取物对水稻纹枯病菌、黄瓜炭疽病菌和番茄青枯病菌等有明显的抑制生长作用[ 6~ 8] 。本研究以银杏为材料， ， Abstract Endophytic fungi is a kind of special fungus growth in living plant tissue,whitch research is relatively small,it grew up in special environment, with medicinal value and old long or have regional characteristics of plants in general can be new endogenous bacteria was isolated, while new species and rare species can provide prospects for search for new bioactive substances. Ginkgo is characteristics of rare plants in our country, has“ Living fossils” good name. Ginkgo rarely infection diseases and pests during growth, its life is very long, so it is good to dig up the potential new type of endophytic fungi, this study collected hubei province of hubei liberal arts college in ginkgo biloba leaves and branches of xiangyang city,, using chromatography separation of endophytic fungi, bacteria screening with antimicrobial properties and antioxidant activity of strains and preliminary identification of morphological characteristics; Significant activity of three bacteria antimicrobial properties and stable does not produce robe, studied the morphological and molecular analysis, optimize the culture conditions and medium composition, antibacterial activity of the fermentation substances into miseparation and structural identification, for further industrialized production application work provide dhi and basis. Ginkgo, also known as ginkgo, is our country unique rare and precious