中度嗜盐菌Vibrio sp.K1-L一种噬菌体的分离、鉴定.doc

存档日期： 2011年06月 存档编号： 本科生毕业论文（设计） 论 文 题 目：中度嗜盐菌Vibrio sp.K1-L一种噬菌体的分离、鉴定 姓 名： 院 系： 生命科学学院 专 业： 生物技术 年 级、班 组： 指 导 教 师： 徐州师范大学生命科学学院印制 中度嗜盐菌Vibrio sp.K1-L 一种噬菌体的分离、鉴定 指导教师： (徐州师范大学生命科学学院，江苏 徐州 221116) 摘要：用从盐湖水中分离到Isolation and Characteraztion of a Phage infecting moderately halophilic Vibrio sp.K1-L Youkeji Supervisor:Wang Ziyuan (School of Life Sciences, Xuzhou Normal University, Xuzhou,Jiangsu,221116,China) Abstract: A moderately halophilic bacterial strain Vibrio sp.K1-L，isolated from Yuncheng Saline was used as indicator to scan the water sample from the same location for phage detection. One phage, desed as was isolated. Physiological and biochemical characterization of this phage were conducted. The Investigation of phage genome and structure proteins were performed. This phage is capable of binding on the surface of indicator cells of Vibrio sp.K1-L and was clearly demonstrated by scan electron microcopy. Key words : Phages；Saline；moderate halophiles. 引言 3 1 材料与方法 3 1.1 供试材料 3 1.2配置培养基材料 4 1.3实验器材 4 1.4嗜盐菌噬菌体的获得 5 1.5噬菌体的验证 5 1.6噬菌体的删选及纯化 6 1.7氯仿耐受性试验 6 1.8噬菌体的大量培养 7 1.9噬菌体的过滤及浓缩 7 1.10对噬菌体浓缩液进行SelfinoseTM 4B琼脂糖凝胶过滤层析 7 1.11噬菌体DNA的电泳 7 1.12噬菌体蛋白质SDS—PAGE凝胶电泳 8 1.13噬菌体电镜观察