HAPSITE_ER_顶空操作培训.ppt

* Concentric needle, if an air sample was withdrawn without replacing the volume, it would create a vacuum within the vial. 同心针头, 如空气样品被取出后未替代容积,将在样瓶中产生真空. * NOTE: Sample heat up time is not built into the method. 注: 样品升温时间未建立于方法中. * NOTE: Assembly does not spin completely, it will need to be rotated to the left or right depending on its location. 注: 组件并不完全自旋, 根据它的位置需要左旋或右旋. * Preparing the Blank/Samples-Syringe Techniques准备空样/样品-针管技术 Draw the plunger back until there is a 1 μL plug of liquid between the 1 μL and 2 μL mark 抽回柱塞直到有 1 微升液体在 1 微升与 2 微升标记之间 Plunger will be drawn back to ~4 mL 柱塞被抽回至~4 毫升 Inject this liquid into a waste vial 将此液体注入废瓶中 Disregard small air pockets that may appear near the plunger 不管柱塞附近是否出现小空气泡 Repeat this same procedure for sample preparation 为样品准备重复此同样顺序 * Inserting Blank and Purge Vial into the HSS 将空样和冲净样瓶插入 HSS 中 Open the top lid on the Headspace 打开顶空的顶盖 Lift the needle assembly 提升针头组件 Place the standard blank into a well 将标准空样置入槽中 Place an empty vial into another well 将空的样瓶放入另一个槽中 This is the Purge Vial 这是冲净瓶 * Inserting Blank and Purge Vial into the HSS 将空样和冲净样瓶插入 HSS 中 Center the needle over the septum and gently insert it into the vial for the standard blank 为标准空样对中隔膜上的针头, 将它插入样瓶 Needle guides can be used to help position the needle针头导向有助于针头定位 Close the top lid 盖上顶盖 Allow the blank to heat for 15-20 minutes 让空样加热 15-20 分钟 * Running Analyze 运行分析 Touch RUN ANALYZE 按运行分析 Nitrogen from the headspace needle and bubble into the sample