微量血清的快速质谱分析及其在肝硬化诊断中的初步应用.pdfVIP

现代科学仪器 第5期2011年10月 98 Modern Scientific Instruments No.5 Oct. 2011 微量血清的快速质谱分析及其在肝硬化诊断中的初步应用 1 1 1 1 2 *2 *1 鄢飞燕 　王 姜 　贾 滨 　 陈焕文 　魏益平 　徐建中 　丁健桦 1 （东华理工大学/江西省质谱科学与仪器重点实验室 江西南昌 330013； 2 南昌大学第二附属医院心胸外科 江西南昌 330006） 摘　要　采用自制的纳升级微量取样器，在无需样品预处理下，对纳升级的血清进行了快速表面解吸常压化学电离质谱 分析，并耦合多元统计主成分分析方法，进行数据处理。实验结果表明，该方法能快速的检测微量的血清样品，通过主成分方法， 能很好的将肝硬化患者和正常人区分开，为肝硬化的早期诊断提供了新思路和新方法。 关键词　表面解吸常压化学电离质谱;微量取样;主成分分析;肝硬化 中图分类号　O657.63 Rapid Differentiation of Trace Serum Samples for Diagnosis of Liver Cirrhosis Using Surface Desorption Atmospheric Pressure Chemical Ionization Mass Spectrometry 1 1 1 1 2 2 *1 Yan Feiyan , Wang Jiang , Jia Bin , Chen Huanwen , Wei Yiping , Xu Jianzhong , Ding Jianhua 1 ( Jiangxi. Key Laboratory for Mass Spectrometry an d Instrumentation, East China Institute of Technology, Nanchang, P.R. China; 2The Second Affi liated Hospital to Nanchang University, Nanchang, 330006) Abstract A method based on a nanoliter level sampling technique and desorption atomsperic pressure chemical ionization mass spectrometry(SDAPCI-MS) was developed for the rapid analysis of trace serum without any sample pretreatment. Principal component analysis (PCA) was performed for the data-processing.The results showed that pattern recognitions at the metabolites fi ngerprinting level for diagnostic analysis of liver cirrhosis serum samples are achieved successfully, a novel idea- SDAPCI-MS method is provided for early liver cirrhosis diagnosis. Key words Surface desorption atmospheric pressure chemical ionization mass spectrometry; Nanoliter level sampling; Princi