As2O3对ACC-2移植瘤血管生成的影响.docVIP

  As2O3 对 ACC-2 移植瘤血管生成的影响# 高丽，张斌** （哈尔滨医科大学附属第二医院口腔颌面外科，哈尔滨 150086） 5 10 15 摘要：目的 研究三氧化二砷（As3O2）对人腺样囊性癌 ACC-2 裸鼠移植瘤生长及血管生成 的抑制作用，探讨其抗肿瘤的作用机制。方法 通过建立 ACC-2 裸鼠移植瘤模型，将 28 只 荷瘤鼠随机分为 4 组：阴性对照组（生理盐水即 NS）、阳性对照组[5-Fu10 mg/(kg· d)]、低 剂量组[As2O32.5mg/(kg· d)]、高剂量组[As2O3 5.0 mg/(kg· d)]。连续经腹腔注射用药 10 天。 测量肿瘤的体积和重量，采用 HE 染色光镜观察，免疫组织化学法检测 VEGF、MVD 的表 达并进行统计学分析。结果 高、低剂量 As2O3 组移植瘤的瘤体积、瘤重均低于两对照组（NS 组和 5-Fu 组），且高剂量 As2O3 组抑瘤最明显，瘤体积抑制率为 52.17﹪, 瘤重抑制率为 56.04﹪（P0. 01）。在移植瘤表面，与两对照组相比，高、低剂量 As2O3 组血管密度减小， 管径变细。高、低剂量 As2O3 组抑制移植瘤 VEGF 的表达、降低 MVD 值，高剂量 As2O3 组抑制作用最明显。与两对照组比较以及高、低剂量 As2O3 组之间比较差异均有统计学意 义(P≤0.01)。结论 As2O3 对人腺样囊性癌 ACC-2 裸鼠移植瘤及血管生成有明显抑制作用， 其作用机制可能为降低 VEGF 表达及 MVD 值，此抑制作用与剂量有关。 关键词：三氧化二砷；腺样囊性癌；裸鼠；移植瘤；血管生成 中图分类号：R 739.8 20 Effects of Arsenic Trioxide on ACC-2 Xenogiogenesis in Nude Mice Gao li, Zhang bin (Department of Oral and Maxillofacial Surgery,The 2nd Affiliated Hospital of Harbin Medical 25 30 35 40 University,Harbin,150086) Abstract: Objective This study was to investigate the inhibitory effect of Arsenic Trioxide(As2O3)on salivary adenoid cystic carcinoma-2 (ACC-2) cell xenografts and angiogenesis in nude mice .Methods The nude mice with ACC-2 cell xenografts were randomly distributed into four groups, control normal saline(NS), 5-Fu group[5-Fu10 mg/(kg·d)]，low-dose group[As2O32.5mg/(kg· d)],and high-dose group[As2O3 5.0 mg/(kg· d)]0.2ml NS or drugs were given by intraperitoneal injection for 10 days. Tumor volume and weight were measured, the microstructure was observed by HE staining, the expression of VEGF and MVD were detected by immunohistochemistry. Result The average tumor volume was significantly smaller and the average tumor weight was significantly lighter in As2O3 groups than in control groups, especially in the high-dose group，the inhibitory rate were 52.17﹪and56.04﹪（P0.01）Compared with the control group, MVD of treatment group marked decreases, the pipe diameters are smaller. The expression levels of VEGF and MVDwas significantly lower in As2O3 group