DNA疫苗对实验性大鼠牙槽骨吸收的保护作用研究.docVIP

  DNA 疫苗对实验性大鼠牙槽骨吸收的保护 作用研究# 郭红梅，王喜军，杨丕山* 5 10 15 20 25 30 35 40 （山东大学口腔医学院，济南 250012） 摘要：目的：以重组质粒 pIRES-fimA:IL15 作为 DNA 疫苗，观察其对 P. gingivalis 引起的实 验性牙周炎的保护作用。方法：将疫苗经滴鼻免疫 Wistar 大鼠，用 P. gingivalis 接种大鼠口 腔，PCR 方法检测接种后大鼠口腔中的 P. gingivalis 菌。实验结束时，取大鼠颌骨去除软组 织后测量釉牙骨质界到牙槽嵴顶的距离作为牙槽骨吸收情况，并做组织切片观察组织学改 变。比较实验前后各组大鼠体重变化，并取大鼠主要脏器做组织切片观察有无明显病理改变。 结果：在 P. gingivalis 引起的大鼠实验性牙周炎模型上，pIRES-fimA:IL15 经滴鼻免疫牙槽 骨吸收量显著小于未免疫组，同时也小于 pIRES-fimA 免疫组。实验结束时，pIRES-fimA:IL15 和 pIRES-fimA 免疫组大鼠唾液中未检测到 P. gingivalis。在实验前后大鼠体重增加各组之间 没有显著差异，大鼠主要脏器也没有明显病理改变。结论：基因疫苗 pIRES-fimA:IL15 能够 对 P.gingivalis 引起的实验性牙周炎提供安全有效的保护。 关键词：牙周炎；牙龈卟啉单胞菌；FimA；IL-15；DNA 疫苗 中图分类号：R781.4+2 Protection of DNA vaccine against alveolar bone loss in rats Guo Hongmei, Wang Xijun, Yang Pishan (Stomatology School, Shandong University, Jinan 250012) Abstract: The co-expression plasmid pIRES-fimA:IL15 was used as a DNA vaccine to investigate the protection against P.gingivalis-induced alveolar bone loss. After immunized with the vaccine, the rats were infected with P. gingivalis. PCR was performed to detect P. gingivalis in saliva. Alveolar bone loss(expressed by distance from the cenentoenamel junction to the crest of the alveolar bone) was measured directly after soft tissue around tested teeth was removed. The results showed that no P. gingivalis were detected in saliva of pIRES-fimA:IL15 and pIRES-fimA immunized groups at the end of the experiment and P.gingivalis-induced alveolar bone loss in sham-immunized rats was significantly greater than pIRES-fimA:IL15 and pIRES-fimA immunized rats. Alveolar bone loss in pIRES-fimA:IL15 immunized rats was significantly less than in pIRES-fimA immunized rats. The weight increase at the end of experiment among groups had no significant difference. The HE stain of the main internal organs showed no evident pathological manifestations. The results suggest that nasal immunization with pIRES-fimA:IL15 in rats provided more protection against P.gingivalis-induced alveolar bone loss. Key wo