PD-1在慢性HCV感染者CD4+T细胞不同亚群中的表达.docVIP

  PD-1 在慢性 HCV 感染者 CD4+T 细胞不同亚群 中的表达# 沈弢，郑佳佳* 5 10 15 20 25 30 35 （北京大学医学部基础医学院病原生物学系） 摘要：目的 分析 PD-1 在慢性 HCV 感染者 CD4+T 细胞亚群的表达情况，以及 PD-1 表达水平与 HCV 血浆病 毒载量和酶学指标的相关性。 方法 采用流式细胞术对 19 例慢性 HCV 感染者和 17 例健康对照者进行了外 周血 CD4+T 细胞亚群和表型分析，及各细胞亚群 PD-1 表达水平的测定。同时将细胞亚群的比例，PD-1 表 达水平与 HCV 病毒学指标及酶学指标进行了关联性分析。 结果 慢性 HCV 感染者 Na?ve T 细胞明显低于健 康对照者，而 TEM 细胞和终末效应 T 细胞明显高于健康对照者。PD-1 在慢性 HCV 感染者中各 CD4+T 细胞亚 群和总 CD4+T 细胞中的表达水平升高。慢性 HCV 感染者 PD-1 表达水平升高与 HCV 病毒学指标及酶学指标之 间无明显相关性。 结论 本研究系统分析了 HCV 长期慢性感染者体内外周血 CD4+T 细胞亚群分布特征。结 果显示分析患者外周血 CD4+T 细胞表面成熟和活化抑制标志对评价宿主细胞免疫水平提供了一个很好的参 考指标。 关键词：基础医学；PD-1；Na?ve T 细胞；TCM 细胞；TEM 细胞；效应 T 细胞 中图分类号：[R394.5] The study of PD-1 expression on different CD4+T cell subsets in chronic HCV infected patients Tao Shen1, Jiajia Zheng2 (1. Department of Microbiology, Peking University Health Science Center; 2. Department of Microbiology,Peking University Health Science Center) Abstract: Objective To evaluate the PD-1 expression on different CD4+T cell subsets in chronic HCV infected patients, and investigate the correlation of PD-1 abnormal activating expression on CD4+T cell with HCV virological and enzymological indicators. Methods A study of the percentage of CD4+T cell subsets and MFI of PD-1 expression on total CD+T cells and its subsets were performed by multi-parameter flow cytometry in 19 chronic HCV infected individuals and 17 healthy control volunteers. Additionally, we studied the correlations between PD-1 expression and HCV Virological and enzymological indicators. Results The percentage of Na?ve T cell subsets was lower but TEM and Effector T cell subsets were higher in 19 chronic HCV infections compared to healthy control. The level of PD-1 in total CD4+T cells and four CD4+T cell subsets increased dramatically in chronic HCV infections. There was no significant difference between the upregulation of PD-1 and the HCV virological and enzymological indicators. Conclusion The distributional pattern of peripheral CD4+T cell subsets and the characteristics of