PLGA_Col_nHA纳米纤维膜的制备、表征及生物相容性研究.docVIP

  PLGA/Col/nHA 纳米纤维膜的制备、表征及 生物相容性研究# 徐红珍，苏俭生** 5 10 15 20 25 30 35 40 （口腔生物医学及转化医学实验室，同济大学附属口腔医院修复科，上海，200072） 摘要：目的 研究聚乳酸-羟基乙酸共聚物/胶原/纳米羟基磷灰石（PLGA/Col/nHA） 纳米纤维膜材料与大鼠骨髓间充质干细胞的体外生物相容性，探索其作为骨组织工程支架 的可行性。方法 采用静电纺丝技术制备 PLGA/Col/nHA 纳米纤维膜材料，并通过透射电 镜、扫描电镜以及接触角测量对其进行形貌及亲水性能表征。取第三代大鼠骨髓间充质干 细胞(BMSCs)，接种于上述纳米纤维膜支架，构建细胞－膜支架复合体并进行成骨诱导培 养。通过 MTT 法检测细胞在材料表面的增殖情况；扫描电镜观察细胞在膜表面的形态变化 及黏附、增殖情况；碱性磷酸酶含量测定检测其诱导成骨性能。结果 PLGA/Col/nHA 纳米 纤维膜展现良好的亲水性能，且 BMSCs 在其表面生长良好，增殖较快，碱性磷酸酶活性表 达较高。结论 PLGA/Col/nHA 纳米纤维膜材料具有良好的生物相容性及成骨诱导性能，可 作为骨组织工程支架进一步应用于体内骨缺损修复实验研究。 关键词：静电纺丝； 纳米纤维膜；骨髓间充质干细胞；骨组织工程； 生物相容性 中图分类号： R318.08 preparation and Characterization of PLGA/Col/nHA Electrospun Nanofiber Membrane XU Hongzhen, SU Jiansheng (Laboratory of Oral Biomedical Science and Translational Medicine, Department of Prosthodontics, School of Stomatology, Tongji University, Shanghai, 200072) Abstract: Objective To construct an electrospinning PLGA/Col/nHA nanofiber membrane seeded with rat bone marrow mesenchymal stem cells (BMSCs), and explore its feasibility for bone tissue engineering. Methods PLGA/Col/nHA nanofiber membrane as scaffold was produced by electrospinning technique, and its morphology and hydrophilic characteristics were investigated by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and contact angle measurements. The third generation of rat BMSCs was harvested and seeded onto the scaffolds to develop a cell-scaffold complex, and cultured for osteogenesis. Cell proliferation of the material surface was tested by MTT; the morphology, adhesion and proliferation of cells seeded onto scaffolds were observed by SEM; alkaline phosphatase activity was detected to determine osteogenic induction potential. Results PLGA/Col/nHA nanofibers membrane materials showed good hydrophilic, could promote the proliferation of BMSCs and higher expression of alkaline phosphatase activity. Conclusion PLGA/Col/nHA nanofibers material has good biocompatibility and osteogenic induction propertie