TGF-β1诱导人前列腺癌PC3细胞上皮间叶化的研究.pdfVIP

网络出版时间：2013-05-24 15:02 网络出版地址：/kcms/detail/31.2035.Q1502.001.html 中国细胞生物学学报 Chinese Journal of Cell Biology 2013, 35(6): http ://www.cj TGF-β1诱导人前列腺癌PC3细胞上皮间叶化的研究 陈丹扬 刘 浩 王 昊 王险峰 杜 军* ( 中山大学药学院微生物与生化药学实验室, 广州510006) 摘要 上皮细胞间叶化(epithelial-mesenchymal transition, EMT)与肿瘤侵袭转移密切相关, 转 β β 化生长因子- (transforming growth factor-beta, TGF- ) 已被证实为肿瘤EMT的主要诱导剂。该研究 旨在探讨TGF-β1对人前列腺癌PC3细胞EMT发生的诱导作用。经TGF-β1处理的PC3细胞, 在相差 倒置显微镜下观察到细胞形态由上皮型向间叶型转化; 细胞划痕和Transwell 实验检测细胞的迁移 能力较对照组显著增强; Real-time PCR 、Western blot和细胞免疫荧光验证TGF-β1能上调间叶型标 记蛋白的表达及下调上皮型标志蛋白的表达。进一步发现, TGF-β1对转录因子Snail的表达没有明 显的影响, 但是能促进Snail的入核, 干扰Snail能逆转TGF-β1对PC3细胞EMT发生的诱导作用。该 研究表明, TGF-β1能够介导Snail的入核而激活Snail, 促进前列腺癌PC3细胞的发生。 关键词 TGF-β1; 上皮细胞间叶化; Snail; 前列腺癌 TGF-β1-induced Epithelial-mesenchymel Transition in Human Prostate Cancer Cells PC3 Chen Danyang, Liu Hao, Wang Hao, Wang Xianfeng, Du Jun* (Department of Microbial and Biochemical Pharmacy, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China) Abstract It has been proved that epithelial-mesenchymal transition (EMT) is correlated with tumor inva- sion and metastasis. Transforming growth factor-beta (TGF-β) has been identified as a main inducer of EMT. The aim of this study is to investigate the effects of TGF-β1 on EMT in human prostate cancer PC3 cells. We found that TGF-β1 treatment could induce morphological alteration of PC3 cells from epithelial