抑制素B对精子发生障碍的诊断参考价值.pdfVIP

362 国际生殖健康/ 计划生育杂志2009 年 11 月第28 卷第 6 期 J Int Reprod Health蛐Fam Plan ，November 2009 熏Vol. 28熏No. 6 综 述 抑制素B 对精子发生障碍的诊断参考价值 蔡文娟综述 谷翊群审校 【 要】 抑制 B 是睾丸分泌的一种糖蛋白激 ，受垂体卵泡刺激 (FSH)特异调控，具有昼夜分 泌节律性并随年龄增加而变化。成年男性抑制 B 几乎全部来自于睾丸，且其组装过程大部分在生精细 胞内完成，而后释放到循环和精浆，因此血清和精浆抑制 B 水平与精子发生同步。由于使用高特异性抗 体， 联免疫吸附法（双抗体 心ELISA 法）可精确测定具有生物活性的抑制 B ，满足科研和临床的需 要。抑制 B 的浓度在精子发生障碍时会出现变化，通过测定其浓度可对精子发生状态作出评估，在鉴别 梗阻性与非梗阻性无精子症方面也具有较高的特异性与敏感性。 【关键词】 抑制 B ；精子发生；卵泡刺激 ；无精子症 Application Value of Inhibin B Levels in Diagnosis of Spermatogenetic Malfunction CA I W e n -j uan ，GU Y i-q un. G raduate School of Peking Union Medical College ，Be ij ing 100730，China（CA I W en-j uan ）；De partme nt of Male Clinic Research ，National Research Institute for Family Planning，Be ij ing 100081 ，China（G U Y i-qun ） 【Abstract 】 Inhibin B is one of glycoprotein hormones secreted by testis and regulated specifically by follicle stimulating hormone from pituitary. Its secretion has circadian rhythm and it changes with aging. Inhibin B in male adult almost derives from testis and its assemble process is mostly finished in the spermatogenic cells. Then it is released to the circulation and seminal plasma. As a result ，the levels of inhibin B in the serum and seminal plasma are synchronized to spermatogenesis. Enzyme linked immunosorbent assay （Double-antibody Sandwich Method of ELISA ）can determine precisely biological active inhibin B due to the use of the high specific antibody ， which could meet the needs of scientific research and clinical diagnosis. The concentration of inhibin B changes with spermatogenetic malfunction. Therefore ，spermatogenesis status can be assessed by measurement of inhibin B concentration. It has higher sensitivity and specificity in differential obstructive and non-obstructive azoospermia. 【Key words 】 Inhibin B ；Spermatogenesis ；Follicle