一个与水稻条纹叶枯病抗性基因紧密连锁分子标记鉴定.pdfVIP

农业生物技术学报 Journal of Agricultural Biotechnology 2009,17 (1): 144~147 ·研究论文· 一个与水稻条纹叶枯病抗性基因紧密连锁的分子标记的鉴定* 周 彤，王 磊，程兆榜，陈 洁，范永坚，周益军** （江苏省农业科学院植物保护研究所，南京210014） 摘要：针对水稻条纹叶枯病粳型(Oryza sativa ssp. j aponica)抗源镇稻88 尚无可供育种利用的分子标记的现状，以镇稻88 和感病品种武育粳3 号配组构建F 作图群体。在采用单分蘖鉴定和苗期接种鉴定方法对F 群体和F 家系分别进行抗性遗传 2 2 2:3 分析的基础上，通过标记分析表明，RAPD 标记OPO11 与镇稻88 中抗性基因紧密连锁，表现为共分离。使用该标记对徐稻3 号等3 个镇稻88 衍生抗性品种进行分析，结果表明，RAPD 标记OPO11 可应用于镇稻88 与武育粳3 号组合及其衍生组合的 分子标记辅助选择。 关键词：水稻条纹叶枯病；水稻品种；抗性；分子标记 中图分类号:S188 文献标识码:A 文章编号:1006-1304(2009)01-0144-04 Identification of A Marker Closely Linked to Rice Stripe Disease Resistance Gene in a Japonica Rice Variety ZHOU Tong, WANG Lei, CHENG Zhao-bang, CHEN Jie, FAN Yong-jian, ZHOU Yi-jun** （Institute of Plant Protection ，Jiangsu Academy of Agricultural Sciences, Nanj ing 2 10014 , China） Abstract ：Markers related to the gene of rice stripe resistance in Oryza sativa ssp.j aponica Zhendao88 were screened. The inheritance of rice stripe resistance in F lines and F lines from the cross Zhendao 88/ Wuyujing No.3 were studied using tiller inoculation and inoculation 2 2:3 at the seeding stage, respectively. A marker OPO11, which was coseperated or completely linked to rice stripe disease resistance gene in Zhendao 88, was obtained by RAPD analysis. The amplification results of OPO11 primer in several resistance j aponica varieties, such as Xudao No.3, originated from Zhendao 88, were the same as that of Zhendao 88. The OPO11 marker linked to the rice stripe resistance gene in Zhendao 88 could be used in marker-assisted selected program for rice stripe resistance breeding.