攝護腺與血清檢驗PSAFPSA的應用.ppt

Time Resolved Amplified Cryptate Emission Eu 3+ cryptate (Eu 3+ tribipyridine diamine) Amplified Energy Transfer RECOVERY OF THE SPECTRA DONOR-ACCEPTOR An Internal Reference KRYPTOR前所未見極佳之CV值 以AFP為例 Within-assay c.v.(%) 2.0 Between-assay c.v.(%) 6.0 The reading of the signal occur 50μ seconds after the excitation up to 450μ seconds which allows to eliminate the fluorescence of potential parasites and free XL665 conjugate. TRACE technology uses a signal with a non destructive emission which can be measured continuously. The unique advent allows the early detection of elevated concentrations. TRACE allows kinetics measurements and so the rapid evaluation of the concentration range of samples during the first minutes of incubation. Heterogeneous method: Assay requiring a solid phase and a separation step between bound components and free components prior to the measurement. Homogeneous method(Direct method): Biochemical assay performed in a single step process,in a single solution. Immunometric assay The donor: Europium cryptate is conjugated to a highly specific antibody. The acceptor: the fluorescence XL665 is conjugated to a second highly specific antibody. Competitive assay The donor: Europium cryptate is conjugated to a highly specific antibody. The acceptor: the fluorescence XL665 is conjugated to the antigen Heterogeneous method: Assay requiring a solid phase and a separation step between bound components and free components prior to the measurement. Homogeneous method(Direct method): Biochemical assay performed in a single step process,in a single solution. Immunometric assay The donor: Europium cryptate is conjugated to a highly specific antibody. The acceptor: the fluorescence XL665 is conjugated to a second highly specific antibody. Competitive assay The donor: Europium cryptate is conjugated to a highly specific antibody. The acceptor: the fluorescence XL665 is conjugated to the antigen Eu3