一个亚洲棉MYB家族新基因克隆及特征及分析_杨召恩.pdfVIP

2013,46(1):195-204 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2013.01.023 1 / / 455000 2 466000 Cloning and Characterization of a Noval Gene of MYB Family from Gossypium arboreum L. 1 1 1,2 1 1 1 YANG Zhao-en , YANG Zuo-ren , LIU Kun , LIU Chuan-liang , ZHANG Chao-jun , LI Fu-guang 1 ( Cotton Research Institute, Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton Biology/Key Laboratory for Cotton Genetic Improvement, Ministry of Agriculture, Anyang 455000, Henan; 2Key Laboratory of Plant Genetics and Molecular Breeding of Zhoukou Normal University, Zhoukou 466000, Henan) Abstract: ObjectiveThe objective of this study is to clone a novel gene of MYB family (GaMYB2) from Gossypium arboreum L., to analyze its expression patterns, and to characterize the deduced protein of this gene. MethodThe full length of GaMYB2 was cloned by RACE and RT-PCR techniques, the gene sequence obtained and the putative amino acid sequence was analyzed by bioinformatics software. Its expression patterns were analyzed by real-time PCR under normal and PEG6000 stress respectivelywhich simulated drought tolerance.ResultThe GaMYB2 full length cDNA sequence is 1 117 bp, and the ORF is 840 bp, which encode a deduced protein including 279 amino acid residues. Blastp reseach revealed 40.50% to 68.20% similarities between GaMYB2 and other reported species, such as Oryza sativa (BAA23338.1) and Tritic