一个玉米类ABC1基因ZmABC1-10克隆及其对镉等非生物胁迫应答.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2010, 36(12): 2073−2083 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2010.02073 Cloning of an ABC1 -like Gene ZmABC1-10 and Its Responses to Cadmium and Other Abiotic Stresses in Maize (Zea mays L.) GAO Qing-Song, YANG Ze-Feng, ZHOU Yong, ZHANG Dan, YAN Cheng-Hai, LIANG Guo-Hua, * and XU Chen-Wu Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology / Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China Abstract: Cadmium is a non-essential heavy metal that is extremely toxic to plants and animals. Previous studies have shown that several proteins associated with the Activity of the bc1 complex (ABC1) protein family participate in plant responses to cadmium. Here we presented the cloning and characterization of an ABC1 -like gene, ZmABC1-10, from maize (Zea mays L.). The full-length 2 519 bp cDNA of maize ABC1-10 gene contained an open reading frame (ORF) of 2 250 bp encoding a membrane-binding pro- tein with a predicted localization in the chloroplast. A promoter scan detected numerous cis-elements implicated in abiotic stress, light, and phytohormone responses. Expression profile analysis indicated most expression of this gene occurred in green tissues. Cadmium treatment revealed that expression of this gene could be induced and was correlated with plant development. In addition to cadmium, ZmABC1-10 expression was also affected by a broad range of abiotic factors, such as ABA, H O , drought and dark- 2 2 ness. A total of 19 members of maize ABC1 family were identified with the B73 maize genomic sequence. Phylogenetic analysis using 148 ABC1 proteins from 8 re