骨髓间充质干细胞定向诱导成类软骨细胞的实验研究_李冬梅.pdfVIP

Progress of Anatomical Sciences 2004, 10(4) : 311~ 314 1 1 1 2 1 1 李冬梅 , 金连弘, 张 宇,李呼伦 , 刘慧雯, 张宝东 ( 11 ; 21 , 150086) = 体外诱导骨髓间充质干细胞(MS s) 向软骨细胞表型分化, 探讨其分化的条件, 为其作为软骨 组织工程种 细胞提供实验基础 取第二代成年大鼠骨髓间充质干细胞, 实验组用无血清培养液诱导; 对 照组用含10% 胎牛血清的培养液自然分化Ò型胶原免疫组化甲苯胺蓝染色透射电镜检测其分化情况统计 学分析不同时间不同接种密度的软骨分化率 诱导后的MS s 具有软骨细胞特点, 不同诱导时间不同接 种密度的Ò型胶原免疫组化阳性率有显著差异诱导14 d 为最佳, 诱导率与细胞接种密度成正相关 成年 大鼠骨髓间充质干细胞在无血清培养基能向软骨细胞方向转化 = 骨髓间充质干细胞; 软骨; 分化; 成年大鼠 = R336 = A = 1006- 2947(2004)04- 0311- 04 Experimental Study of Differentiation of Marrow Mesenchymal Stem Cell into Chondrocyte-like Cells LI Dong-mei, JIN Lian-hong, ZHANG Yu, et al ( Department of Histology and Embryology, Harbin Medical University, Heilongjiang Harbin 150086 hina) = Abstract Objective Induction of bone marrow mesenchymal stem cells ( MS s) of adult rat in vitro was per- formed to explore the possibility of MS s being cartilage seed cell in tissue engineering. Methods MS s of the second passage of adult rat were divided into test group induced by free-serum HG-DMEM and control group induced by HG- DM EM with 10% fetal bovine serum respectively. ollagen type Ò immunohistochemical staining, toluidine blue stain- ing, transmisson electron microscope were performed to detect differentiation. Statistical analysis of the MS s differentia- tion was deployed under various induction time and various plating density. esults Induced MS s have characteristics of the chondrocyte, the positive percentage of collagen type Ò immunohistochemical staining was different statistically be- tw een various in