MiR_138对MCF_7细胞端粒酶活性及端粒稳定性的调控作用.pdfVIP

2010 8 August 2010 30 8 Basic Clin icalM edicine Vol. 30 No. 8 : 10016 32 5( 2 010 ) 0 80 82 605 M iR138MCF7 1 2 1 1 1 1* 1* 石 嵘 , 姜 立, 高 洋, 周珏宇, 丁大鹏 , 郑文岭 , 马文丽 ( 1 , 510515; 2, 510080) : M iR138( hTERT ) , MCF7 M CF7 M iR138 , MTT , 48 h RTPCR hTERT TRAP assay , 53BP1 FISH 48 h, M iR138 MCF7 hTER T 218( 2- Ct ), 26 , 53BP1 ( Foci), 2062% 155% M iR138 hTERT M CF7, : M iR138; ; ; M CF7 : Q 756 : A The regulation of telom erase activity and telomere stability byM iR138 inMCF7 cells 1 2 1 1 1 1* 1* SH I Rong , JIANG L i, GAO Y ang , ZHOU Jueyu , D ING Dapeng, ZHENG W enling , MA W enli ( 1Institute of Genetic Eng ineering, SouthernM edicalUniversity, Guangzhou 510515; 2Guangdong General H ospital, Guangzhou 510080, China) Abstract: Objective To study the regulation mechanism of telomerase activity ofM iR138 by targeting hTERT as the dow nstream molecu lar, and its effect on telomere stab ility in breast cancer cell line MCF7. M ethods M iR 138m mi icsw ere transiently transfected into breast cancer cell lineM CF7. MTT methodw as applied for detecting the effect on cell proliferation. The hTERT expression level of the cellsw as detected by realtmi e RTPCR and te lom erase activity w as checked by TRAP assay 48 h after transfection. M eanwh ile, the cells w ere smi u ltaneously checked by 53BP1 antibody mi munofluorescent staining and telomere FISH stain ing. Results The hTE