愈肾颗粒对肾纤维化大鼠HGFBMP-7表达影响的机制研究_吕静.pdfVIP

第 27 卷 第 12期 Vol. 27 N o. 12 2 0 09年 12月 CH INESE ARCH IVES OF TRAD IT OI NAL CH INESE M ED CI INE Dec. 2 0 0 9 HGF BMP- 7 吕 静,庞立健 (, 110032) : 目的:探讨愈肾颗粒防治肾纤维化大鼠机制研究方法: 大鼠按照性别体重随机分为空白组, 模 型组愈肾颗粒组洛 丁新组运用腺嘌呤灌胃造模的方法建立实验性肾纤维化大鼠模型,采用光学显微镜免疫 组织化学方法,观察检测各组肝细胞生长因子 HGFBM P- 7的表达, 24h后给药, 观察各实验组 21天, 28天, 42 天肾组织HGFBMP- 7表达情况的变化并采用病理图像分析系统,半定量分析 HGFBMP- 7含量表达; 结果: 在 28天42天, 洛 丁新与愈肾颗粒两 个治疗组的表达明显高于模型组同一实相, 彼此之间无明显差异, 都与模型 组均有明显统计学意义, P 0. 01或P 0. 05; 在 21天42天两 个治疗组的表达明显高于模型组同一实相, 彼此 之间无明显差异, 都与模型组均有明显统计学意义(P 0. 01或P 0. 05); 结论: 愈肾颗粒有效提高 HGFBM P - 7蛋白水平的过低表达,从而改善肾纤维化, 潜在的延缓了肾纤维化进程 :愈肾颗粒;肾纤维化; HGF; BMP- 7 : 285. 5 : A : 1673- 7717( 2009) 12- 2659- 03 Yu shenkeli on R enal F ibrosis in R at HGF, BM P - 7 Expression S tudy of the M echanism LU Jing, PANG Li-Jian (Affiliated Hospital of LiaoningUniversity ofT raditional ChineseM edicine, Shenyang 110032, Liaoning, China) Abstrac:t O bj ectiv e: Themore granular control of renalmechanisms of renal fibrosis in rats.M ethods: ats according to sex, were randomly divided into blank group, model group, yushenkeli group, Lotensin group. Adenine gavage using modeling method of establishing expermi ental ratmodelof renal fibrosis. Opticalm icroscopy, mi munohistochem icalmeth- ods, observation and testing of each group ofhepatocyte growth factorHGF, BM P- 7 expression in. 24 hours after adm in- istration was observed in all expermi ental groups 21 days, 28 days, 42 days renalHGF, BM P- 7 expression changes. And the use of pathological mi age analysis system, sem i- quantitative analysis ofHGF, BMP- 7 expression levels. R e- sults: In the 28d, 42d, Lotensin particleswithmore renal expression of the two