野生茄子(Solanumtorvum)抗黄萎病相关基因StoVe1的克隆与分析.pdfVIP

63 8 植物生理学通讯 第42 卷第4 期，2006 年8 月 野生茄子(Solanum torvum)抗黄萎病相关基因Sto Ve1 的克隆与分析 1,* 1,* 1 1 2 2 1,** 史仁玖 殷　 王忠 陈敏 黄权生 黄乐平 杨清 1 2 南京农业大学生命科学学院，南京2 100 9 5 ； 新疆农业科学院核生物技术研究所，乌鲁木齐83 00 0 0 提要 采用RT-PCR 和RACE 技术从野生茄子中扩增克隆到一个抗黄萎病相关基因，命名为Sto Ve1，其cDNA 全长3 400 bp ，含有3 153 bp 的完整开放阅读框，编码1 051 个氨基酸，该基因编码的蛋白序列与刚果野茄、类番茄和番茄Ve1 编 码的氨基酸序列同源性分别为82%、81% 和80%，且有很高的功能区段保守性。将该cDNA 全长序列提交GenBank ，登 陆号为DQ020574 。半定量PCR 表明该基因为组成型表达，在根中表达最多，叶中最少。 关键词 野生茄子；Sto Ve1 基因；克隆；表达 Cloning and Analysis of Full-length cDNA of Sto Ve1 Gene from Solanum torvum 1, * 1,* 1 1 2 2 1,** SHI Ren-Jiu , YIN Yue , WANG Zhong , CHEN Min , HUANG Quan-Sheng , HUANG Le-Ping , YANG Qing 1Colleg e of L if e Sciences, Nanj ing Ag ricultural University , N anj ing 210095, China; 2Institute of Nuclear B iotechnology, X inj iang A cademy of Ag ricultural Sciences, Urumqi 830000, China Abstract The full-length cDNA of a gene associated with verticillium wilt disease resistance, named Sto Ve1, was amplified and cloned from Japanese Solanum torvum by RT-PCR and RACE. The cDNA contained 3 400 bp with an open reading frame (ORF) of 3 153 bp and encoded 1 053 amino acid residues. The deduced amino acid sequence showed 82% homology to SaVe 1 from Solanum aethiop icum, 81% homology to SlVe 1 from Solanum ly cop ersicoides and 80% homology to Ve 1 from Ly cop ersicon p eruvianum and also highly conserved domains of function . This gene has been registered in GenBank with accession number DQ020574. Semi- quantitive RT-PCR analysis indicated that Sto Ve1 expression was different in roots, stems and leaves, and richer in roots, poor in leaves. Key words Solanum torvum; Sto Ve1 gene; cloning; expression 黄萎病(verticillium wilt)是一种由大丽轮枝菌 torvum)来自日本，对黄萎病具有极强的抗性。试 ( Verticillium dahliae)和黑白轮枝菌( Verticillium 验根据番