刺槐根系可溶性蛋白SDS_PAGE分离方法的改进.pdfVIP

2011, 26( 1) : 128~ 130 Journal of Northwest Forestry University S SPAGE * , , ( , 712100) :在利用常规S SPAGE 方法分离刺槐根系可溶性蛋白的过程中, 将样品处理液中的S S 巯基乙 丙三 的含量均增加50% 这样改进后的方法得到了分离效果明显较常规方法好的 电泳图谱经分析得出: 常规S SPAGE 方法中样品处理液的S S 和巯基乙 浓度不足以充 分溶解刺槐根系中的可溶性蛋白, 丙三 的浓度也不足以将样品很好地沉聚于点样槽底部该试 验为植物组织可溶性蛋白的S SPAGE 分离提供了一套效果较好的参考方法 :刺槐根系; 可溶性蛋白;S SPAGE; 改进 :S792.27 : A :2011 Methodological Improvement of Seperating Soluble Protein by S SPAGE from Robiniap seudoacacia Root * CHEN Mingtao, ZHAO Zhong , Quan Jine (K ey Labor atary of Environment and Ecology in Western China, M inistry of Education, N orthwest F university , Yangling, Shaanxi 712100, China) Abstract: An improvement was made in the conventional method S SPAGE for separating soluble protein from the roots of Robiniap seudoacacia by increasing the content of S S, mercaptoethanol and glycerine to 50% . The separating effect of improved method was much better than the conventional one. Causes of the improvement were discussed: in the conventional method, the concentrations of S S and mercapto ethanol were not high enough to completely dissolvethe solubleprotein, and the concentration of glycerine was also not high enough to precipitate the sample. Key words: Robiniap seudoacacia root; soluble protein; S SPAGE; improvement [ 13] 1 , [4] 1. 1 S SPAGE 2009 7 , S SPAGE 2 a [59] , 2~ 3 cm , , (Robiniap seudoacacia) ,- 70 , 1. 2 ( 1) : 0. 062 5 mo