葛根素对低氧性肺动脉高压_r_n大鼠肺动脉压力及结构的影响.docVIP

葛根素对低氧性肺动脉高压大鼠肺动脉压力及结构的影响 扬州市第一人民医院呼吸科（江苏扬州　225001）　　黄玉民 陶玉坚 ［摘要］ 目的：观察大鼠在低氧状态下出现的肺动脉高压以及肺小动脉结构的重建，以及葛根素对这两个因素的干预效应。方法：雄性SD大鼠24只，随机分为对照组、低氧组和葛根素干预组。低氧组和葛根素干预组在常压低氧舱内建立肺动脉高压模型，葛根素干预组大鼠每天腹腔注射葛根素500mg/kg，测定各组平均肺动脉压，并取大鼠左肺沿肺门横断取材，石蜡包埋切片，进行HE染色，观察肺小动脉形态学变化。结果：①对照组和葛根素干预组大鼠平均肺动脉压均明显低于低氧组[( 15.01士1.47)， (20.43士2.86)，（29.74士2.32)，p0.01]。②对照组大鼠肺小血管管壁较薄、管腔较大，低氧组大鼠肺小动脉普遍增厚，管腔狭窄，葛根素干预组的肺小动脉管壁及血管管腔狭窄程度较低氧组显著减轻。结论：①慢性缺氧可导致肺动脉壁增厚、管腔狭窄等血管重建病理改变。②葛根素能在一定程度上抑制低氧肺动脉高压的形成和发展及肺血管结构的重构。 关键词：葛根素；肺动脉高压；低氧/低氧性 Intervention effects of puerarin on the change of pulmonary vascular pressure and structure in hypoxic pulmonary hepertension rats. Huang Yu-Min, Tao Yu-Jian. Department of Respiratory Medicine, Yangzhou First People’s Hospital, Yangzhou city 225001, Jiangsu Province, China. [Abstract] Objective: To investigate occurrence of pulmonary hepertension and the remodeling of pulmonary arteriolar structure in rats under hypoxia, and the interventional effects of puerarin on the two factors. Methods: 24 male SD rats were randomly allocated into 3 groups: contral group, hypoxic group and puerarin interventional group(n=8). Rat models of pulmonary hypertension were established under discontinuous hypoxia in the normobaric hypoxic chamber for two weeks. Intraperitoneal injection of puerarin (500mg/kg) was given to the rats in the puerarin interventional group every day immediately before hypoxia, and the others received injection of saline of the same volume. Average pulmonary artery pressure was determined with pressure sensor. The left lungs of rats taken along the hilum of lung, slices were embedded with olefin and stained with hematoxylin-eosin to observe the morphological changes of pulmonary small artery. Results: ① The average pulmonary artery pressure was obviously lower in the control group and puerarin interventional group than in the hypoxic group [(15.01士1.47), (20.43士2.86), (29.74士2.32)mmHg, P0.01]. ② The pulmonary small artery vascular wall was thinner and vascular cavity was big