Survivin和p53在非小细胞肺癌中的表达及其临床意义.docVIP

Survivin和p53在非小细胞肺癌中的表达及其临床意义 井昶雯，吴建中，马蓉，王卓，曹海霞 江苏省肿瘤医院（临床肿瘤实验中心：井昶Expression of survivin and p53 in non- small cell lung cancer JING Chang-wen , WU Jian-zhong , MA Rong, WANG Zhuo, CAO Hai-xia Affiliated Cancer Hospital of Nanjing Medical University, Nanjing 210009, P.R. China Expression of survivin and p53 in non- small cell lung cancer [Abstract]Objective: The aim of the study is to detect the expression of survivin and p53 in non- small cell lung cancer and their clinical significance and correlation. Methods: Survivin mRNA expression was detected by real-time fluorescence quantitative PCR. Immunohistochemical EliVision method was used to detect the expression of survivin and p53. Results: 76.7% of survivin mRNA expression in tumor tissues was higher than that in their adjacent mucosa. The positive expression rate (74.4%) of survivin in tumor tissues was higher than that (16.7%) in adjacent mucosa tissues. The positive expression rate (60%) of p53 in tumor tissues was also higher than that (13.3%) in adjacent mucosa tissues. There was non-statistical difference between expressive of survivin and histological type, clinical stage, differentiation and lymph node metastasis. There was positive correlation between expression of survivin and p53. Conclusion: The expression of survivin and p53 in non-small cell lung cancer is higher than that in adjacent mucosa tissues and not related to histological type, clinical stage, differentiation and lymph node metastasis. There is a relationship between expression of survivin and p53. [Key words] non-small cell lung cancer, survivin, p53 Survivin和p53在非小细胞肺癌中的表达及其临床意义 【摘要】目的：方法：实时定量PCR的方法检测其中30非小细胞肺癌组织和对应的癌旁组织中mRNA的表达，应用免疫组织化学法检测0例非小细胞肺癌组织中表达, 与病理关系。结果：Survivin蛋白在癌组织阳性表达率（74.4%）显著高于癌旁组织16.7%（χ2=24.7，P 0.001）。p53在NSCLC癌组织阳性表达率（60%）也显著高于癌旁组织13.3%（χ2=12.1，P 0.001）。Survivin表达与非小细胞肺癌的病理类型、分化程度、肿瘤分期以及是否有淋巴结转移无统计学意义的相关性（P 0.05）。Survivin表达与p53表达呈正相关（χ2=4.72，P 0.05）。结论：[1, 2]。非小细胞肺癌（non- small cell lung cancer , NSCLC）是最常见的肺癌类