中空纤维膜电泳富集测定水中的Cr（Ⅵ）.docVIP

中空纤维膜电泳法富集水中的Cr(Ⅵ) 包建民，刘晓静，李优鑫* (天津市现代药物传递及功能高效化重点实验室，天津大学药物科学与技术学院，天津 300072) 摘 要本文建立了一种中空纤维膜电泳快速富集水中CrO42-(Cr(Ⅵ))的方法。将装有去离子水的中空纤维膜垂直放入含CrⅥ)的样品溶液中，正电极插入中空纤维膜内，螺旋形负电极环绕于中空纤维膜外侧，在两电极间施加电压，在电场作用下水中的Cr(Ⅵ)经中空纤维膜膜孔进入膜内的去离子水中。施加电压、搅拌和富集时间是影响富集倍数的主要参数，对三个条件进行考察，确定最佳富集条件为：施加电压20 V，搅拌速度600 r/min，富集时间15 min。结果表明，应用本方法富集水中的Cr(Ⅵ)并进行测定，富集，富集倍数高达约30倍，能显著提高水中Cr(Ⅵ)的富集速度和检测灵敏度。 关键词中空纤维膜；电泳；铬(Ⅵ) Enrichment of Cr (VI) in water by Electrophoresis with Hollow Fiber Membrane BAO Jian-min, LIU Xiao-jing, LI You-xin *(Tianjin Key Laboratory for Modern Drug Delivery and High-EfficiencySchool of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China. 300072) Abstract: This paper established a rapid enrichment method of Cr (Ⅵ) in water by electrophoresis with hollow fiber membrane. Hollow fiber membrane filled with deionized water was put into the sample solution containing Cr (Ⅵ) vertically and the positive electrode was inserted into the hollow fiber membrane lumen with spiral-shaped negative electrode surrounding around the outside of the hollow fiber membrane. When voltage was applied between the two electrodes, Cr (Ⅵ) in water would be pushed into deionized water filling the lumen of the hollow fiber membrane by the electric field. Applied voltage, stirring rate and enrichment time were the main factors that affected enrichment factor and the optimum enrichment conditions: applied voltage 20 V, stirring speed 600 r/min, enrichment time 15 min were determined after experiment. This method was able to enrich Cr (Ⅵ) in water quickly in 15 minutes and significantly enhance enrichment factor up to 30 times or so. Keywords: Hollow fiber membrane; Electrophoresis; Chromium (VI) 铬是广泛存在于自然界的一种元素，其在自然界中以多种形态存在，它的价态可以从+ 2 价到+ 6 价，其中最稳定形态是三价和六价。不同价态会产生不同的生理作用，Cr(Ⅲ)是人体必须的微量元素，而Cr(Ⅵ)则具有致癌毒性，其毒性比Cr(Ⅲ)大100倍[1]。它可使血红蛋白转变为高铁血红蛋白，并可干扰体内的氧化、还原和水解过程。常接触大剂量六价铬会引起接触部位的溃疡或造成不良反应。摄入过量的六价铬会引起肾脏和肝脏受损、恶心、胃肠道刺激、胃溃疡、痉挛甚至死亡[2]。然而，由于工业生产的发展，Cr(Ⅵ)广泛存在于环境中，特别是电镀、制革纺织等工业废水的排放，造成了严重的水污染，因此对Cr(Ⅵ)的监测受到环境