CharacterizationofBetulaplatyphyllagenetranscripts：白桦基因转录产物的表征.docVIP

Characterization of Betula platyphylla gene transcripts associated with early development of male inflorescence Molecular Biology Reports Lei Xing, Xue-mei Liu* Lei Xing, College of life sciences, Northeast Forestry University, Harbin, 150040 Corresponding author: Xue-mei Liu, Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Northeast Forestry University, Harbin, 150040 Tel: 86 451Fax: 86 0451E-mail: liuxuemei@nefu.edu.cn Supplementary table 1: Primers used in RT-PCR and qRT-PCR Name Sequence ME56F 5‘TTCGGAGCCATTGGTGAG’3 ME56R 5‘GGATTGAAGGAGCCAGAGT’3 ME108F 5‘TTCTTAGGAGCCAAACAT’3 ME108R 5‘ACATTTGGACGCTGCATA’3 ME206F 5‘GCCACCCTTTACTTTATG’3 ME206R 5‘ACACCACGCTTTCCTCTG’3 ME310F 5‘GCTAAATCCCAAGACAGG’3 ME310R 5‘GTAAGAATCCAGCAGAGG’3 Action-F 5‘CATCTCTGATCGGAATGGAAG’3 Action-R 5‘AGATCCTTTCTGATATCCACG’3 Supplementary table 2: relevant homologies of ESTs of cDNA-AFLP in the database ESTa Accession NO. Size(bp) Putative function species Homology(%) ME9 HS107072 138 hypothetical protein Vitis vinifera 93 ME21 HS107073 205 short-chain dehydrogenase/reductase (SDR) family protein Ricinus communis 58 ME23 HS107074 199 Putative uncharacterized protein At1g08630 Vitis vinifera 97 ME26 HS107075 255 Large subunit ribosomal protein 1 Koerneria sp. RS1982 43 ME31 HS107076 145 hypothetical protein TTHERMTetrahymena thermophila 78 ME35 HS107077 279 Glutamate receptor 2.2/Ligand-gated ion channel 2.2 Populus trichocarpa 72 ME38 HS107078 230 Putative uncharacterized protein At5g45410 Vitis vinifera 97 ME40 HS107079 250 disease resistance protein (NBS-LRR class), putative Vitis vinifera 87 ME44 HS107080 863 Putative uncharacterized protein Arabidopsis thaliana 98 ME45 HS107081 361 U-box domain-containing protein Ricinus communis 98 ME47 HS107082 284 VHS domain-containing protein / GAT domain-containing protein Vitis vinifera 97 ME48 HS107083 254 F-actin-capping protein subunit beta/CapZ-beta Ricinus communis 31 ME51