病毒性心肌炎病程中肿瘤坏死因子和FasmRNA表达的变化.pdfVIP

2005 28 4 291 Fas mRNA 陈元美 王少敏 吴士尧 ! ( V MC) (T NF) Fas mRNA , VMC B 3 ( CV B ) 3 VMC 714 28 , ( RTPCR) T NF Fas mRNA , , V MC T NF Fas mRNA T NFmRNA 7 , 14 d , 28 d ; Fas mRNA 7 , 14 d , 28 d V MC T NF Fas mRNA T NF, Fas ! ; ; Fas Changes of tumor necrosis factorand Fas mRNA in murine viral myocarditis CH EN Yuanmei, WA N G S ha o min , W U S hiy ao . D ep artment of Card iology , Shanghai N inth P eop le∀ s H osp ital , Shanghhai Se ond M ed i al University , S hang hai 2000 11, China Abstract! Obj ective T o study t h chang s of tumor n crosis factorand Fas mRNA in murin viral myo cardit is ( V MC) , and to apprais th ir rol s and im plications in VMC. Methods Viral myocarditis mod l was construct d by intrap riton al inoculation with CV B solution. Th xpr ssion l v ls of myocardial T NF and 3 Fas mRNA in VMC mic w r studi d by RTPCR, and myocardial histopathologic chang s w r obs rv d. Re sults Compar d w ith th normal control group, th xpr ssion of T NFand Fas mRNA incr as d r markably . Th p ak xpr ssion of T NFmRNA r ach d at day7 aft r inoculat ion, and high l v l w as maintain d v n aft r day14, but d cr as d significantly on day 28. T h xpr ssion of Fas mRNA incr as d on th 7th day aft r th in oculation, and r ach d th p ak aft r t h 14th day and st ill show d xpr ssion on th 28th day . Conclusions T h xpr ssion of m yocardial T NFand Fas mRNA incr as obviously w hil T NFplay s a rol in t h inflammatory r action and myocardial damag , Fas t ak s part in myocardial n crosis of VM C. ( Shanghai M d J, 2005,