基因芯片在黄曲霉毒素生物合成相关基因差异表达分析中的应用研究.pdfVIP

208 2009, Vol. 30, No. 17 食品科学 ※生物工程 1 2, 胡 娜 ，许 杨 * (1. 江西科技师范学院生命科学学院，江西南昌 330013 ；2. 南昌大学中德联合研究院，江西南昌 330047) 为寻找与黄曲霉毒素生物合成密切相关的基因，本研究采用诱导产毒培养的方法培养寄生曲霉AS3.4407 ， 获得产毒与不产毒两种性状的菌丝体。应用基因芯片技术和RT-PCR 方法筛选与黄曲霉毒素生物合成相关的差异表 达基因。研究结果表明产毒菌丝与不产毒菌丝中与产毒相关的部分基因存在差异表达。芯片扫描的结果与RT-PCR 的扩增结果一致，均显示有6 个基因存在表达差异，它们分别是aflA 、aflE 、aflF 、aflR 、aflT 和aflX 。本研究 证实aflR 基因表达的改变与黄曲霉毒素的产生密切相关。 寄生曲霉；黄曲霉毒素；基因芯片；差异表达基因 Application of Gene Chips in Analysis of Differential Expressions of Genes Involving in Aflatoxin Biosynthesis 1 2, HU Na ，XU Yang * (1. College of Life Science, Jiangxi Science and Technology Normal University, Nanchang 330013, China； 2. Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, China) Abstract ：The aflatoxin (AF) production was induced in Aspergillus parasiticus AS3.4407 by shifting the cultures from non- conducive to conducive medium and aflatoxin B1 (AFB1)-producing and non-AFB1-producing mycelia were obtained and lyophilized. Subsequently, differential expressions of genes involving in the AF biosynthesis were screened using gene chips and RT-PCR. The results from gene chips were consistent with those from RT-PCR amplification, suggesting differential expres- sions of six genes including aflA, aflE, aflF, aflR, aflT and aflX. This study proves that the differential expression of aflR is closely related to the aflatoxin production. Key words ：Aspergillus parasiticus ；aflatoxin ；gene chips ；differential gene expression 中图分类号：Q789 文献标识码：A 文章编号：1002-6630(2009)17-0208-04 分子遗传学分析表明，黄曲霉与寄生曲霉的黄曲霉