37例老年患者胰十二指肠切除术手术风险因素探讨（附6例死亡病例分析）.pdfVIP

维普资讯 大 医 学版 南 学学报 SoutheastUniv SciEdi JSoutheastUnivMedScEd){2一007,Nov；26(6)’4．l3。．416 4·l3· (Med Earthworm fibrinolyticenzymeenhancesradiosensitivityof ． humanesophagealcarcinomacelllineECA109 CHEN Hong，HUANG J ，TUWen—yong ，ZHANG Zhi—guo ，CHEN Bao—an (1．DepartmentofGastroenteroloyg，ZhongdaHospital，SoutheastUniversity，Nanjing210009，China；2．DepartmentofTumor Radiotherapy，theSecondHospitalofNanjing，AffiliatedtotheSchoolofMedicine，SoutheastUniversity，Nanfing210003， China ；3．InstituteofBiochemistryand Immunology，NanjingAgrciultureUniversity，Nanjing210095，China； 4．DepartmentofHemotology，ZhongdaHospital，SoutheastUniversity，Nanjing210009，China) Abstract：Objective Tostudytheradiosensitivity—enchancingeffectofearthwormfibrinolyticenzyme(EFE)on humanesophagealcarcinomacelllineand itsrelatedmechanism．M ethods TheesophagealcarcinomacelllineEC A109wasusedintheexperiment．MTFassaywasperfomr edtoanalyzetheinhibitoryratioofthecellline，theclonogenic assaywasusedtodetemr inecellsurvivalfraction，theflow cytometerwasusedtocheckcellcycledistributionandthe RT—PCRwasusedtodetemrinethegeneexpressionofseleniumdependentglutathioneperoxidase(SeGPx)．Results TheproliferationofcelllineECA109wassuppressedbyEFEinvitro．Theinhibitoryconcentrationof50％ (IC50)was 3．8ukU ·ml～．EFE coulddefinitelyenhanceradiosensitivityofECA 109 cells．Thesensitivity．enchancement．ratiowas 1．37for0．1ukU ·ml～EFE and1．59ofr0．3ukU ·ml～ ofEFE respectively．EFEabrogatedtheG，／M arrestinduced byy—ray．Meanwhile，theexpressionofSeGPxmRNAdecreasedaftertheEFEor(and)y—raytreatment．Conclusion EFEmightbeapotentialradiosensitizerofresophagealcarci