GC测定砷(Ⅲ)在小鼠血浆和血细胞中的浓度及药动学研究.pdfVIP

GC 测定砷( ul ) 在小 鼠血浆和血细胞 中的浓度及药动学研究 李士敏，，朱亚尔’，李俊，(1. 浙江大学分析测试中心，杭州310029;2. 浙江省台州市药品检验所，浙江台州318000) 摘要: 目的 建立血装和血细胞中砷( 111) 的气相色谱测定方法，考察小鼠静脉注射砷( 111) 溶液后血浆和血细胞中的药动学情 况。方法 血装样品经6% 高氯酸去蛋 白，二筑基丙醇( BAL) 衍生化后 ，苯萃取 ，浓缩后用苯溶解后进样 ，血细胞样品则直接 衍生化后经苯苹取 、浓缩、再溶解后进行 GC 分析。采用该方法测定给小鼠静脉注射砷( 111 ) 后血浆和血细胞 中砷( III ) 浓度 ， 计算主要的药动学参数。结果 血桨和血细胞中砷( 111) 分别在0.037 5 - 2.40 和0.03125 -- 1. 00 mg L- 内‘线性关系良好， 两者的tvz。分别是7. 178 和2.531mn,t 1/2。分别为108.731和 146.236 mn,AUC。一二则分另，]为72.334 和112.239 mg - mn L- 1。结论 本实验建立的小鼠血样中砷的气相色谱测定法设备简单方便、方法灵敏可靠，砷在小鼠血浆和血细胞 中的消除 很快，没有蓄积的倾 向，而血细胞 中砷的浓度要高于血浆 中的浓度。 关键词: 砷( 11I ) ; 气相色谱法;血浆;血细胞 中图分类号:R969 文献标识码:A 文章编号: 100 1 - 2494 (2007) 14 一1084 - 04 Determ nat on of As ( II I ) n Plasma and Red Blood Cell s by GC and Its Phar macok net c Analys s n M ce LI Sh -mn ,ZHU Ya-er ，Ll Jun2( 1. Centre of Test ng andAnalyss,Zhej ang Unversty,Hangzhou 310029，Chna; 2. Tazhou Inst tute f or Drug Control , Ta zhou 3 18000 , Ch na ) ABSTRACT ;OBJECTIVE To develop a GC method for the determnat on of As( 111) n mouse plasma and red blood cells and to nvest gate the pharmacok net cs of As ( I11 ) n m ce after an ntravenous nj ect on of arsen te solut ons. METHODS After be ng deprotenedby6%HC104,dervat zedwth2，3-dmer- captopropanol (BAL) ，extractedbybenzene,dr edbyN2，andredssolved n 0. 1 mL benzene ,plasma samples were separated 场 GC. Red blood cells were d rectly der vat zed and nj ected after the same process. Mouse blood samples were collected at d fferent t me po nts 场 ext rpat ng eyeball after ntravenous nj ect on ,determ ned by establ shed “determnat on methods. The ma n pharmacok net e parameters were determned thereby. RESULTS Cal brat on curves of As( 111) n plas ma and red blood cells were both l near n the ranges of 0.037 5 一2.40 and 0.03125 一1.00 mg ·L一t respect vely.t 2, were respect vely 7. 178 and 2.531mn,t ，二。were 108.731and 146.236 mn,AUC。一。were 72. 334 and 112.2