E-test法检测幽门螺杆菌对3种抗生素的耐药性.pdfVIP

Dis，February25，2013，V01．33，No．1 鱼匾塑垡壅盘查!!!!笙!旦笙!i鲞釜!塑!!!!Dig ·61· ·论著· E—test法检测幽门螺杆菌对3种抗生素的耐药性 高承霞严祥韩俭王琼 摘要：目的 了解幽门螺杆菌(Hp)对临床常用3种抗生素的耐药性，以指导临床合理用药。方法收 集上消化道疾病患者胃黏膜标本，分离培养、鉴定出42株Hp临床菌株，采用E—test(Epsilometest)方法测 定3种抗生素对Hp菌株的最低抑菌浓度(MIC)。结果 42株临床分离的Hp菌株对甲硝唑、克拉霉素、 阿莫西林的耐药率分别为64．3％、14．3％和0。结论Hp对甲硝唑耐药率高，故甲硝唑不应作为根除Hp 感染的一线药物；Hp对克拉霉素耐药率较高，应在药敏监测下选择使用；Hp对阿莫西林敏感，可作为根除 Hp感染的主要药物。 关键词：幽门螺杆菌；抗生素；耐药性；最小抑菌浓度 DOI：10．3969／j．issn．1673—534X．2013．01．018 ofhelicobactertothreeantibiotics E‘lestmethodGAO N NG Resistivity pyiori investigatedby Cheng—xia，YAXiang，WA Lanzhou Qiong．Departmentofgeriatrics，FirstHospitalof UfBiologyPathogens，LanzhouUniversity，Lanzhou(730000)，China The of tothree usedantibioticswere the Abstract：0bjectiveresistivity Hp commonly investigated，thusguiding reasonablemedicationinclinicalcases．MethodsThe werecollectedfrom mUCOUSmembraneof with specimens gastric patients disease．Fromthese 42 strainswereisolated，cultivatedandidentified．The uppergastrointestinal specimens，total Hp minimumantibacteriaIconcentrations(MIC)ofstrainstothreeantibioticswereexaminedwithE-test．ResultsThe Hp average of42 0 antibioticsresistancerates strainswereestimatedaS64．3％，14．3％andfor Hp amoxicillin Since isolatedis tometronidazole，thosemetronidazole— resistant respectively．Conclusionsclinically Hp highly containedmedicinesshouldnotbeselectedasfirst—choicetreatm