台湾地区芽胞杆菌种类多样性及地理分化.docVIP

台湾芽胞杆 田潇娜1，2，刘波2*，刘国红1，2，唐建阳2，林营志2 （1 福建农林大学 福州 350002，2 福建省农业科学院农业生物资源研究所 福州 350003） 摘要：芽胞杆菌（Bacillus）是一种革兰氏阳性菌，能够在多种不良环境下存活且具有特殊功能，在生产实践中已被广泛应用。为了丰富芽胞杆菌资源，作者首次对台湾地区芽胞杆菌多样性进行调查台湾8个市县20个土样154株芽胞杆菌利用16S rRNA基因序列进行测序鉴定，获得21种芽胞杆菌引入多样性指数对台湾地区从北向南各县市芽胞杆菌种类多样性及其分布规律分析16S rRNA基因序列苏云金芽胞杆菌（Bacillus thuringiensis）为台湾的广分布芽胞杆菌种类，在各个地区均有分离到；阿氏芽胞杆菌（Bacillus aryabhattai）、蜡状芽胞杆菌（Bacillus cereus）和炭疽芽胞杆菌（Bacillus anthracis）为常见分布芽胞杆菌种类；台湾地区存在着特异性分布的芽胞杆菌种类丰井氏芽胞杆菌（Bacillus asahii）、嗜气芽胞杆菌（Bacillus aerophilus）、延长芽杆菌（Lysinibacillus macrolides）、黄海芽胞杆菌（Bacillus marisflavi）和Bacillus oceanisediminis在大陆的分布中未见报道 关键词：台湾；芽胞杆菌；多样性Diversity investigation of Bacillus-like species in Taiwan Tian Xiao-na1,2, Liu Bo2*, Liu Guo-hong1,2, Tang Jian-yang2, Lin Ying-zhi2 1. Fujian Agriculture and Forestry University, Fuzhou 350002, China；2. Agricultural Bio-resource Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China Abstract:Bacillus-like species, a Gram-positive bacterium, can survive in a variety of adverse environmental and has a special function. It has been widely used in the production practice. In order to rich the Bacillus resources, for the first time the author to investigate the diversity of Bacillus-like species. Bacillus-like species was separated by plate dilution coating method from 20 soil samples in 8 counties and cities in Taiwan, and got 154 stains, being distinguished by 16S rRNA gene sequencing and 21 of Bacillus-like species were identified. Statistical of Bacillus-like species about the sample collection and areas were analyzed, the author introduces the diversity index and system clustering method, and diversity of Bacillus-like species and its distribution pattern for the cities and counties from north to south in Taiwan were analyzed, In addition, of the 16S rRNA gene sequence cluster analysis method was used to investigate the geographic distribution characteristics of 78 stains separated from Taiwan before. Survey results show that the morphological